pCAMBIA-1391质粒图谱及相关信息
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3 \& z" s& z5 e: H载体名称：pCAMBIA-1391
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载体类型：双元载体
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4 I% O: k) i3 J) j& ]% \9 g& Q报告基因：GUS
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抗性基因：kan（菌） hpt2（植物）
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载体图谱：
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载体序列：
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) u5 ]- C" d) r! c载体酶切位点：（Dnaman软件分析）
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List by Site Order
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7 BglII 2908 BglI 5121 BspMI 8710 XhoI
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14 SpeI 3142 BglI 5151 SunI 8712 SciI
1 \& u2 ^* M; ^59 RleAI 3143 XmaIII 5151 SplI 8724 XorII
3 [' ^1 e7 d8 [! V( ^* q
74 BsmI 3143 EagI 5171 XmaIII 8724 PvuI
+ S y, |$ V$ V. ^3 C5 v90 NruI 3143 Eco52I 5171 EagI 8803 ScaI
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90 SpoI 3143 NotI 5171 Eco52I 8829 Eco52I
p' H. [1 Z: y! ^1 s106 BclI 3247 Eco52I 5244 Eco52I 8829 XmaIII
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147 MfeI 3247 XmaIII 5244 XmaIII 8829 EagI
, l: X* C$ K! t9 d7 P; Z1 C296 RleAI 3247 EagI 5244 EagI 8875 BspMII
4 K2 s2 ^2 Z0 w+ f" v8 y9 B3 U
401 SnaBI 3330 BglI 5267 NheI 8875 AccIII
3 k# z/ r1 o0 q/ |574 EcoRV 3341 Eco56I 5347 ClaI 8890 DrdI
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612 MluI 3342 BglI 5360 EagI 9011 AccIII
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619 BspMI 3342 SfiI 5360 XmaIII 9011 BspMII
# \ y y( R% Z/ @638 MscI 3343 NaeI 5360 Eco52I 9024 BstD102I
& n; b( ~: \ v% l/ W1 K; b638 BalI 3345 FseI 5484 MstI 9187 Alw44I
" _. d2 L( v3 I7 ?- b9 K805 EcoRV 3351 SfiI 5515 BspMI 9187 ApaLI
, ^4 R, g6 Q; u; L+ I859 AlwNI 3351 BglI 5580 EcoNI 9190 BsiI
4 d% |9 F- j) P. l901 BspHI 3379 Eco57I 5670 Eco31I 9195 DraIII
X% y# M' B! \" q950 Alw44I 3621 Eco52I 5761 PinAI 9196 ApaBI
' Z* y5 s8 u& S7 }/ X950 ApaLI 3621 XmaIII 5761 AgeI 9268 Tth111I
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965 VspI 3621 EagI 5827 AhaIII 9271 DrdI
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1038 Eco57I 3697 EagI 5827 DraI 9321 NdeI
4 |& o' w5 Z8 p" N% `1090 PvuII 3697 XmaIII 5878 MscI 9362 CspI
( c/ @( I1 q, X1110 AsuII 3697 Eco52I 5878 BalI 9382 BstD102I
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1110 Csp45I 3699 Eco56I 5887 SapI 9399 Eco52I
5 Q) `# {, |& t1137 Bsp1407I 3701 NaeI 5965 EagI 9399 XmaIII
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1275 Alw44I 3812 NdeI 5965 XmaIII 9399 EagI
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1275 ApaLI 3826 XcmI 5965 NotI 9411 PvuI
2 `- Y0 G% p) E9 T& S1286 SspI 4007 AlwNI 5965 Eco52I 9411 XorII
2 }; ]1 p; p4 g3 M( d+ w1310 MluI 4016 Eco56I 5977 BstD102I 9431 PinAI
8 }0 ~% Z: T" j0 @1328 MluI 4018 NaeI 6049 Eco56I 9431 AgeI
+ p- U k4 _! }/ _' |! l) k1349 BspMI 4075 AlwNI 6049 SgrAI 9456 BspMI
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1563 Bsp1407I 4075 PflMI 6051 NaeI 9472 ApaBI
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1734 SpoI 4156 Eco56I 6053 NarI 9488 DraIII
1 t5 z" r9 G/ e- t1734 NruI 4158 EagI 6054 EheI 9489 ApaLI
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1826 NheI 4158 NaeI 6056 BbeI 9489 Alw44I
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1849 Eco72I 4158 XmaIII 6073 RleAI 9548 BspMII
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1849 DraIII 4158 Eco52I 6173 DrdI 9548 AccIII
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1849 PmaCI 4192 AclI 6225 Tth111I 9564 EagI
3 o: l- b2 W2 p1853 XcmI 4198 MscI 6294 Alw44I 9564 Eco52I
" V; ?6 | }$ t, F1859 BstEII 4198 BalI 6294 ApaLI 9564 XmaIII
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1883 PvuI 4204 BglI 6301 NdeI 9669 BsiI
: { S/ P/ C; U' G0 S) d1883 XorII 4245 Eco56I 6303 ApaBI 9712 Tth111I
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1910 AflII 4247 NaeI 6362 SapI 9750 AatII
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2001 NsiI 4288 Eco57I 6411 BstD102I 9787 XmaI
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2081 BssHII 4439 BsmI 6586 DrdI 9789 SmaI
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2105 BssHII 4465 BalI 6651 BsiI 9804 XhoI
% @' Y: X A' l" u6 [2217 DraI 4465 MscI 6792 ApaLI 9806 SciI
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2217 AhaIII 4491 Eco56I 6792 Alw44I 9939 EcoRV
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2264 SphI 4493 NaeI 6894 AlwNI 9969 BbvII
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2295 ScaI 4617 BglI 7026 Eco57I 9969 Bsc91I
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2348 Bsc91I 4617 SfiI 7202 BsmI 9972 BbvII
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2348 BbvII 4675 NotI 7204 NsiI 9972 Bsc91I
. l2 }% r8 ?; D% _2522 BglI 4675 XmaIII 7242 SspI 9979 XmnI
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2585 XmaIII 4675 EagI 7315 EcoRV 10001 BsiI
% |" I% Q, r: @' `8 ]2585 Eco52I 4675 Eco52I 7484 DraI 10197 BbvII
- V" B' K2 f. k# Q% r2585 EagI 4712 Eco56I 7484 AhaIII 10197 Bsc91I
: v7 W! G/ K" x% A5 d; S2 O2587 Eco56I 4714 NaeI 7513 DraI 10246 DrdI
# A/ v" Z+ b9 m: [0 ?( w2589 NaeI 4723 ApaBI 7513 AhaIII 10296 Bsc91I
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2650 BalI 4919 Eco56I 7516 Bsc91I 10296 BbvII
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2650 MscI 4921 NaeI 7516 BbvII 10299 BbvII
0 t( `2 H5 P9 r( P9 o8 c0 _5 S2671 XcmI 4921 Eco52I 7588 EspI 10299 Bsc91I
" h1 h D2 B: h! R5 F7 I2761 Eco56I 4921 EagI 7588 Bpu1102I 10306 XmnI
8 Q- }% X. h" [# q2763 NaeI 4921 XmaIII 7738 BspHI 10328 BsiI
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2811 Eco56I 4923 Eco56I 7844 XcmI 10524 Bsc91I
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2813 EagI 4923 FseI 7880 DraI 10524 BbvII
( c* S$ T1 |: `4 ~- J; `# e% n2 [# B2813 NaeI 4925 NaeI 7880 AhaIII 10573 DrdI
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2813 XmaIII 4929 BglI 8040 AhaIII 10591 BstXI
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2813 Eco52I 4929 SfiI 8040 DraI 10598 ApaI
1 I$ m! n$ k8 w: E2 q2815 Eco56I 4929 RleAI 8096 BsmI 10601 BssHII
# |1 k6 O- R/ \- z5 t2817 NaeI 4932 BalI 8190 Mlu113I 10601 AscI
5 G: E+ S/ l' `2 J" |2817 XmaIII 4932 MscI 8192 SstII 10608 HindIII
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2817 Eco52I 4939 PflMI 8192 SacII 10609 BspMI
4 c0 w+ q( M/ @: x; U5 i2 f6 q2817 EagI 5024 Eco52I 8195 BclI 10620 PstI
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2819 FseI 5024 EagI 8322 ApaBI 10622 SalI
: M6 G% k! A' `* S2842 Eco56I 5024 XmaIII 8352 Tth111I 10628 BamHI
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2844 NaeI 5068 Eco56I 8524 VspI 10633 XmaI
6 |5 E6 ?. a2 q. G' B* a2891 BstD102I 5070 NaeI 8525 XmnI 10635 SmaI
( ~; p) \% d( p* X2908 SfiI 5116 BsmI 8549 ScaI 10638 EcoRI
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Non Cut Enzymes
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Acc65I Asp718I AvrII Bsu36I CvnI Eam1105I
2 t3 S9 e4 N% V: p; k6 r0 ^! DEcl136II Eco47III EcoICRI HpaI I-PpoI KpnI
\ E* ?: _' E$ y5 o$ t5 s' HMstII NcoI PacI PmeI SacI SauI
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SrfI SstI StuI SwaI XbaI
