pBI221质粒图谱及相关信息% F0 @2 [5 k( {- o8 I1 H8 V
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质粒名称：pBI221: L4 B; |2 z* b+ p' Y
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载体类型：双元载体
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抗性：amp9 W7 V1 p3 Y5 ^- w; |" v! j
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报告基因：GUS
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pBI221图谱：" J  Y+ E, ^% l+ ]$ x6 D

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pBI221详细信息及序列：/ ^1 I8 L8 t% o& m5 c
LOCUS       AF502128                5667 bp    DNA     circular SYN 15-MAY-2003
4 O1 |/ N' s- I( LDEFINITION  Transient expression vector pBI221, complete sequence.
: a& q& H+ C4 W7 r. o( h3 z; iACCESSION   AF502128
; q3 ?* n, h% Z% r6 _VERSION     AF502128.1  GI:20454202
% w2 M* V+ q. B1 l/ PKEYWORDS    .
, d7 Q, V  f9 B* ^0 cSOURCE      Transient expression vector pBI221
' S; i& w$ J$ t- r* _  ORGANISM  Transient expression vector pBI221" c' |/ x, B6 f7 Z6 Q/ U
            other sequences; artificial sequences; vectors.
! g* L% U9 h- g% J+ ^REFERENCE   1  (bases 1 to 5667)1 P- u  d% i! Y4 r# f5 i6 K9 V
  AUTHORS   Chen,P.Y., Wang,C.K., Soong,S.C. and To,K.Y.
- {" C# [1 V, j+ ~5 U  TITLE     Complete sequence of the binary vector pBI121 and its application6 c+ Q4 f: O  _7 m6 Z
            in cloning T-DNA insertion from transgenic plants( W9 g1 \. q: S; }( g
  JOURNAL   Mol. Breed. 11, 287-293 (2003)& f  W. ^/ J2 D) O& C/ Z3 c1 x9 j) c
REFERENCE   2  (bases 1 to 5667)) C, w; c* `# K& U6 s8 ~3 ^
  AUTHORS   Chen,P.Y., Wang,C.K. and To,K.Y." J: N- J6 ?4 A, S
  TITLE     Direct Submission
# I; }/ w. n1 w' l0 N3 u, L  JOURNAL   Submitted (12-APR-2002) Institute of Bioagricultural Sciences,
( l1 ^1 k; b  ?4 X$ {8 m            Academia Sinica, Taipei 11529, Taiwan
$ X! l: [' z6 I# Z' }FEATURES             Location/Qualifiers5 F1 S& F# s& ^: j6 V/ ^: k
     source          1..5667. p" Z; Z7 W0 h3 o2 }/ O
                     /organism="Transient expression vector pBI221"
9 q3 p& a; j6 P$ v/ X                     /mol_type="genomic DNA"* e0 e8 G2 W$ G+ b, o$ O! L3 o
                     /db_xref="taxon:193296": A! a- L1 p- _/ f( @$ T' M. q- l8 X. d
                     /note="HindIII-EcoRI fragment (3032 bp) carrying CaMV 35S$ W5 a4 n! k, Q" |1 E# T8 @
                     promoter, GUS coding region and nos terminator was( @' n! v: B) M; ]# B/ y
                     digested from the binary vector pBI121 and ligated with
( k! p; e* h1 z: W- X- p                     cloning vector pUC19, which was previously digested with
" T, l' l, k4 [) U                     HindIII and EcoRI; binary vector pBI121 sequence is
* i" b) U& W. B                     deposited in GenBank Accession Number AF485783; plasmids
9 N4 a* E( y/ G5 ^8 Y- _                     pBI121 and pBI221 were purchased from Clontech5 j+ x* C/ T) `
                     Laboratories, Palo Alto, CA, USA"0 |! ?+ {4 a4 h, t
     promoter        25..859
! r9 @3 l) o' |- w. b$ D                     /note="CaMV 35S promoter"
4 X* P5 @4 O% `     gene            896..2707. w4 L) G2 `* [+ T
                     /gene="gusA"2 z. U# ~0 C3 i; k, ^
     CDS             896..2707' N; U, F* _( O
                     /gene="gusA"( ]1 q! l+ j$ H& \4 q
                     /codon_start=1/ g9 W; s- x/ K  R1 N& x
                     /transl_table=115 a& Y" g/ i' B5 Z
                     /product="beta-glucuronidase"
- ~- o/ ~' g. p% ?                     /protein_id="AAM22211.1"
: f, R0 i9 R; Y1 l9 N& G                     /db_xref="GI:20454203"" Q& C; P9 R2 T/ |5 P# q4 L
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
2 g- `, m# u1 f  R! a/ n                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
6 m7 l- a" @8 ~7 d                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
! o' A7 x; _8 I( j& H. K5 H; h                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
5 d+ N, b: _6 ^2 f                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
3 C* H: L: a9 W3 i! S2 R7 B                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA6 F# a2 ?0 @4 [5 F; n+ C$ y
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
' ~3 B/ ?0 Z5 U' U. C9 e                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD9 _, j5 ?, u7 @2 F5 h( K. q( J7 Q
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD. @2 {2 A& j  T+ |
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN+ b( q- q  m1 L. i  g6 Z
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"+ `0 V9 J, H3 P; s. k
     terminator      2778..30302 }6 Q, h& V9 W1 n6 Q; ^" w, J
                     /note="nos terminator"
4 ^, b  N* c- a6 R; O& G: V     gene            3634..44944 L, L' i$ W' F3 ]- a
                     /gene="amp". N  S& }# J% h% S- r
     CDS             3634..4494& M1 g0 ~6 [! W5 z) j8 r
                     /gene="amp"  |* h3 l" t+ [! Q/ m
                     /codon_start=17 t+ m' N; f! H% ?4 Y
                     /transl_table=11
! U, C9 q2 X1 V/ m                     /product="beta-lactamase"  R# d  i/ n0 j" ^! j, d
                     /protein_id="AAM22212.1"
, P) W6 }! M0 C: p. E  }$ Y                     /db_xref="GI:20454204"
2 a2 h( f% v: p6 |                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
) R* I9 Z: q  t' @7 a                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
6 {+ H9 A. w  W& N$ t                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL  S! Q5 F2 _# I
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
* y& B2 J7 V7 M$ L' z0 a7 l                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA" q* G/ j1 o; t! F; S- |* T. o. ~
                     EIGASLIKHW"9 c8 H1 L5 p, d5 i
     rep_origin      4642..5285) W4 r" e2 [8 [8 M) E5 m/ \+ R
                     /note="pUC19 plasmid replication origin": f8 v. |/ @. }; t6 \
ORIGIN      
  c5 I% x, I4 X+ @( o, d        1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
# t( P  h( M9 d# l" l2 V( x       61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata+ v8 z% E8 X0 s# t# O. s+ s' j
      121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga- M) h4 `: ?4 n& a5 N8 X/ y5 J8 Y
      181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag" S% W- f4 o; l3 ~# T' d# R
      241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
9 e% X) Y1 K) H4 o9 I) r+ C! ?0 b      301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa$ R; y* I$ M5 s
      361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
# u, y/ _) E! u' a2 P1 x      421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
, F. W+ {6 E& E8 ]" A* R      481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
9 ]' K% P, u' Z% N3 a      541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa9 y. o$ W  L6 Q$ |
      601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg# [2 e$ B8 n: L  y6 D5 K
      661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
8 N% L: I% }9 [- `& S+ W- ~      721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
% \, M/ H# `! ~( X+ |      781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
" L& n1 c( D$ e* ^7 S# _# j      841 ttcatttgga gagaacacgg gggactctag aggatccccg ggtggtcagt cccttatgtt
- k$ g3 B* [- M3 @8 W% H2 q- D      901 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
. p# ]0 S6 @7 M2 {      961 tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag
9 `+ b9 Z+ O* K2 R6 E9 S     1021 ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa
% g( V" O7 I" G8 x" I     1081 ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca) q' Y( e0 s0 f& W4 h
     1141 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca; s) q, H5 [- S- K" v) B
     1201 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
/ R: G. M! a0 k     1261 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
$ {' C2 q! }' M     1321 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca' h7 A8 V" ~. l: _
     1381 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac  Q- K$ x0 S6 n3 f9 L% T
     1441 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt" b/ S5 d) \, T9 K5 x  r
     1501 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
8 f: L9 R- A2 _     1561 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
9 c0 H3 q+ Y) A' C; t4 s     1621 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg6 G9 p1 h/ Q  w- I$ ]3 l$ R
     1681 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct: S( r7 u9 b0 {. d
     1741 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
: P. i* D7 A5 f% V  G' {     1801 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
* t" Y/ n* F1 c5 J* E0 X) a     1861 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
* e( ~) y1 G6 x& F     1921 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
2 F4 \/ ]# V1 d/ K- a  l4 ]+ d& _     1981 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg& O5 J/ ~' _) Q( @' {$ c) K
     2041 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca! Y3 {$ w; d1 X. x8 {9 \1 m) C* g9 Z
     2101 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg' Q5 E# a3 }% Z
     2161 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
% D2 g" j% ]- P! d5 C8 b  d     2221 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt" m. Q0 K$ u" O$ U$ K. ?) [
     2281 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
2 t( l4 O( M: T3 T     2341 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat6 ~2 Z9 C  j: k4 T: }, f7 I
     2401 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag% s5 D+ J" k( I' n; J: e. m
     2461 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc6 C5 k: h$ D4 Q. Q; K' c
     2521 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
1 A+ T( \* d' b. n     2581 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
' r! d' |. S, l0 _5 \, y% d     2641 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa8 ~# \' M4 l# G6 J) o9 M4 E
     2701 acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac; ~* K3 X& u; A3 P
     2761 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc$ }: s9 W1 _1 Z* L
     2821 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa& m4 L1 Y3 `# a5 `
     2881 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
& ?. G% N" w: \3 e2 d     2941 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat1 N! o! P7 ?: {6 t" q) h
     3001 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
, `( @/ |% ]: e/ f/ c5 u/ d     3061 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc6 s# U9 m1 j0 I* E& t: D2 l7 g
     3121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
" i9 q. h% q5 S/ p1 A     3181 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca8 I* F4 Q  u. U% J" E
     3241 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4 h5 T0 G1 f- P/ x8 @     3301 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct+ N5 P7 G: i- J
     3361 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca+ ?: v' T; S" y) B2 C& r" X
     3421 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg) Z7 G& D' b% E  O5 M+ h/ f
     3481 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct. p5 x1 G# `& {5 M* @1 w- f
     3541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga6 U4 m" E" k% \5 C5 E$ n4 p# ]
     3601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
4 o2 P+ t! ^, _, e( B& p     3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
2 R/ n5 h4 e3 n; `/ ]6 k     3721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc' a4 v- Z+ K0 q. l  v0 b  T
     3781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact8 e: G* I& v. J7 X  q) b( @
     3841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc8 g* n: t5 T% \; i$ W9 C
     3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag+ I0 x3 m( G* g! h! {
     3961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
7 h; m$ j, Z" I/ a, l( q     4021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
6 ]( Y1 y- m' a& D- V: D5 X3 W     4081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
1 j0 r# u9 S$ h# S. P0 U0 Q     4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
2 W7 m3 L; P0 T  g     4201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg5 g1 l# y* }! C" ~+ h* D* e# V+ T
     4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
2 C" m* ^7 F( H9 Q     4321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
6 k: i. H/ M; u/ J- S5 D0 m9 m  C     4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
9 y% F4 V; e$ {     4441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
' `" |0 w/ P* I     4501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
7 S+ g: n$ D3 F6 K     4561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg1 f4 ?$ g. l" ^/ U+ }
     4621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
% [8 y* {8 `/ j9 w8 w6 k  v: s, _     4681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg- }! a2 D* w) `2 N+ E
     4741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata$ L8 q9 p! ]1 |$ K! }' l1 r& `2 \6 W, @
     4801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca8 G, Q0 v: s/ J7 s: V! c
     4861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag5 X, Z6 J' z, q, |
     4921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
; t8 A7 O/ J0 J     4981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
3 k, `/ E7 z  v9 ]7 ~     5041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
' n# s0 V6 y2 Y     5101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
+ Q3 [9 C. ]- B3 Q* s' _: N     5161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
1 |: L& {  J% m1 X     5221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg% |4 U. s- o$ ?* [$ j( u# A! F
     5281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
0 f: `9 b2 Q( F. v# W     5341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc  s6 M" q) w" q$ C* ]" ]3 W
     5401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
" ]0 |. E/ J; ^  e( r: i4 k! _     5461 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
0 w% O8 {2 C' ?7 p# q     5521 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
3 ^" x( Y" i: J     5581 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
8 w. N. B) i1 H" j. V% ?     5641 ggaaacagct atgaccatga ttacgcc- o8 Q! ~. R! `" e! \' }
//

! J2 A: ]( }7 U" x. \3 V  ^感谢楼上的大哥帮忙！谢谢！

请问在哪去找你需要的质粒的信息啊  多谢