前一段时间找到了这个载体，pcambia1305.1* a7 X  {/ p3 `" ~$ K* Y+ `* F
准备转个基因的启动子，希望对大家有用
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[ 本帖最后由 小宝 于 08-7-23 11:48 编辑 ]

给各全图能怎样啊

让大家看看呗

pcambia1305.1全图及详细特征
4 _/ A" d+ Z2 Q" h7 i. C载体名称：pCAMBIA1305.1
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  A+ Z% v5 `4 s7 l: Y5 Z载体抗性：Kan
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- Y% G" M  K& q# I, I% `- I/ k' C载体宿主：bacteria、plants# J2 L7 U. x* v9 `1 Z2 g6 I" p

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% z+ W. K! o" S# O4 s" h载体图谱：  I3 K1 b+ {+ z7 P# i- C4 f0 E
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载体序列：
, H7 x7 q9 m" S% o7 x& k- c6 LLOCUS       AF354045               11846 bp    DNA     circular SYN 16-APR-2001
8 _* S7 O" X9 b$ MDEFINITION  Binary vector pCAMBIA-1305.1, complete sequence.! k, D, u4 ]/ H, e3 O3 @& E
ACCESSION   AF354045
7 A) B9 R% f5 A. jVERSION     AF354045.1  GI:13540346
, }2 }9 }% j5 ?* gKEYWORDS    .1 s. ^/ S. e8 B$ n7 w+ v
SOURCE      Binary vector pCAMBIA-1305.1: G: E8 A6 m; G) ?! ]# ?( X4 i: V& {
  ORGANISM  Binary vector pCAMBIA-1305.1* n5 i& D9 q4 `! p
            other sequences; artificial sequences; vectors./ k5 j7 J! B) B8 q+ r0 k
REFERENCE   1  (bases 1 to 11846)7 @: ?  n& D5 U/ Z: t$ u. X6 _" B" z
  AUTHORS   Jefferson,R.A., Kilian,A. and Keese,P.K.
+ G6 S& N' t9 O9 a- |  TITLE     Microbial genes for secreted beta-glucuronidases, gene products and
* B1 t4 S+ O. l+ C, |- b3 |6 g            uses thereof( }7 E. _; j% j. J
  JOURNAL   Patent: PCT (PCT/US98/19217)-A 09-SEP-1998;2 Y) J- ~, |$ ^* T0 t1 ~+ J0 L
            CAMBIA; Molecular Technologies; Canberra;. u$ Y0 p* C# B
            Australia;
$ {0 o3 n. T& E7 r& tREFERENCE   2  (bases 1 to 11846)
/ r0 J( a! @3 i8 {" l  AUTHORS   Nguyen,T.A. and Jefferson,R.A.7 l3 X/ z' j0 L  e7 [1 S3 G5 Z
  TITLE     Binary vector pCAMBIA-1305.1* M; ]! N" X( {0 {
  JOURNAL   Unpublished/ F; l9 B4 p3 @6 x* m/ s+ \3 C
REFERENCE   3  (bases 1 to 11846)  y1 k- n! v8 ~3 R; }( a7 x
  AUTHORS   Nguyen,T.A. and Jefferson,R.A.
5 \4 \; h( s) {& R: W; i  TITLE     Direct Submission. Q! D* k$ ~0 [' k# R8 _
  JOURNAL   Submitted (13-FEB-2001) Molecular Technologies, CAMBIA, GPO Box
6 K+ i1 [) p% Q            3200, Canberra, ACT 2601, Australia% r7 ^; O7 b% c1 Z
FEATURES             Location/Qualifiers
9 V' Q4 I! l& \2 D; J% l; @     source          1..11846
; ^6 v, [" T% u                     /organism="Binary vector pCAMBIA-1305.1"$ F. B7 x# P  n6 N$ M) I& i) ]3 b
                     /mol_type="genomic DNA"9 g1 Z# x; m: Y7 L4 y7 c
                     /db_xref="taxon:156490"8 b& ~0 s9 b) ~2 D
     CDS             join(2..16,207..2054)
& {; `/ {( {+ G6 R, i; B                     /note="GUSPlus-His6"
( o/ g6 v0 h2 O# ~                     /codon_start=1: ]. W0 W6 `' h7 @0 c$ P
                     /transl_table=11
1 ?4 l8 [6 ?# Z* s                     /product="beta-glucuronidase"3 w* D8 ]6 C- m! u
                     /protein_id="AAK29426.1"
5 t$ Z' q" x* F/ Q, G+ _+ Z                     /db_xref="GI:13540350"
; f( ?6 M  a- q/ g% M' \7 h                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYE; o5 A, T7 J5 E  d6 |2 C
                     SKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHK
9 t* X: y, ^* E5 n" L+ e3 [                     AIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHE
1 z8 j: G. l( r& w3 a+ |                     EGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQ+ D* H8 Q  ?) T. L9 Q' j
                     GKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTID( s* L% h( G9 W& |, s' \" |
                     VYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWI
- g2 g& B6 l* \$ D1 _3 d                     GANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEK, y7 r$ H/ }; |! R3 n4 ~6 ~
                     IRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQK4 B* U( k! S7 |3 H" ~5 |
                     RPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRC
0 v+ F  \' E5 \/ t% s  @- E* E/ F2 D( o                     PGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFAD
: T+ V$ F, t2 t, H* X- z                     FATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"& _2 f4 ]! a4 `! l- M
     intron          17..206
  K- C, e9 z, L0 S" G& _                     /note="catalase intron"8 \- |; B0 y/ E* }
     polyA_signal    2083..23351 k4 t! ?, p% `5 o
                     /note="poly(A) signal from nopaline synthase", }3 i- c. q" H+ y' j3 Z
     misc_feature    2354..2378
' p/ ]+ i' M! C- c5 a# o( q1 [. ]                     /note="T-DNA right border"( W9 {. X( M0 y
     misc_feature    3439..4439/ J6 r0 l9 d$ n! H& U/ q
                     /note="sta region from pVS1 plasmid"# f+ `* u5 D* e/ E. n
     rep_origin      5032..6032
' R7 y/ T. s5 n& F  S7 C- I                     /note="pVS1 rep"5 ~2 W5 P# m3 W3 P! X  \# z" f
     misc_feature    6442..6702# }; m& x/ \$ t# c' A. M0 a
                     /note="pBR322 bom site"
" \, {) ]$ A$ {& |6 l% O     rep_origin      6842..7122
; |+ O! a; K7 F) P! C                     /note="pBR322 ori"
4 E7 e% S- S% T" I& J$ L     CDS             complement(7413..8207)  O7 Y1 o  r9 |5 C6 n
                     /note="kanamycin resistance gene"* a: j. g, A+ n. p
                     /codon_start=1+ n4 x6 n) S! S4 k
                     /transl_table=111 W5 s& y1 X( ~
                     /product="neomycin phosphotransferase"
, S; x! l! o) }' q- J                     /protein_id="AAK29424.1"' Y( m  f- e% F. j& x4 K% o. A
                     /db_xref="GI:13540348"% C" _/ i) i7 d
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
# }4 ^. Z) `4 J' X9 C8 [                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY0 Z! `& B- O' `! m" u+ s' N4 F
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE- ]' y9 u6 l3 A" i
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
5 ]1 K: m- |. n                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"( T; |( `. y2 x* A  `/ o
     misc_feature    8632..8657
' L7 v+ [/ I+ o5 E                     /note="T-DNA left border"
. N  v. Y; J+ \3 j9 }  s5 _' C     polyA_site      8798..8805
0 ?& g9 e! w0 s' n2 E     CDS             complement(8948..9973)2 A: Q/ h8 B) |. t, r1 k; R, |; h
                     /note="hygromycin resistance gene"
. |$ v- L) {3 s% e                     /codon_start=1
5 A) y9 x  k6 c+ W                     /transl_table=11
/ Q- h; D1 F2 M8 U* U0 g; @                     /product="hygromycin phosphotransferase"2 Y+ U, F  q2 o0 Q  V+ @
                     /protein_id="AAK29425.1"
8 B0 P0 n4 t" I3 @                     /db_xref="GI:13540349"' ?) J! k/ g" _& ]; f2 \. N
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR2 v* R$ r8 b) K# o: z# N
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
3 A8 L9 Y8 h2 }1 |( \" M/ k                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY' F- S; j. Q5 j; Z
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
8 X8 `+ G- [* H& [. g& [7 x$ x8 m                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
( f7 q5 g- \# {2 {+ i0 S! A$ r9 O                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS5 s& p& ]1 f6 ?' E% Q$ k& S! w1 C
                     TRPRAKK"* w, I$ C. @. }3 @% }  ?
     promoter        complement(10009..10816)
; u# o# }4 H) T                     /note="CaMV35Sx2; CaMV35S eukaryotic promoter with/ i5 M8 V, o& B6 L+ x4 {* o) D: v; `
                     duplicated enhancer region"
4 P. P! G' }. T& m  X0 N' _     promoter        10938..11018
. V  i% \& Y" O9 n! P  g; X0 s& ^                     /note="PlacZ prokaryotic promoter"
7 |8 D+ ]9 I4 ^) ^, A! S     CDS             11019..11252
$ |9 z6 s0 B& `  A) h2 ~! k                     /note="beta-galactosidase alpha fragment"* f5 E4 ?0 j6 K! j9 X
                     /codon_start=1
1 s  i& E' e% v2 s0 V! w3 E                     /transl_table=119 P  z3 H8 ~0 r7 F! q6 q
                     /product="lacZ alpha fragment"
% T6 ?- L, C0 u# e- B                     /protein_id="AAK29423.1"
9 M. _" v! N  [                     /db_xref="GI:13540347"
, b3 c8 I9 x4 J5 V6 t/ I! o                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN4 `5 F( L9 O0 C8 V- Q6 Q& ^& u" C
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"; z1 |9 h" f/ g' W$ t
     misc_feature    11033..110895 m8 x  {/ m3 c
                     /note="pUC18 polylinker multiple cloning site"
3 @6 {3 d0 _" s; B" m     promoter        11303..11840
% e8 C8 K6 A/ `* e. M/ \3 N: C                     /note="CaMV35S eukaryotic promoter"
) f2 f- U. ?0 U7 S# D4 Z% N: V& C0 U  IORIGIN      
% m- B" j, B& O# K# _        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
. ]# x0 R: a6 D       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
4 d) h+ ^+ P! R- n7 K# P. ^      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
# W2 \; y% o3 e/ ]      181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
% B1 M1 {' {+ t' N8 G      241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag$ K1 I* Y2 q: Z/ M4 f3 V) k
      301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
% E. j0 w5 u& F  N3 ~      361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
: @/ I# o  Q! @: j4 q) U; i) i      421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
* Z5 h! w3 C& U" k  A% J      481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg: U5 Y7 d5 O$ g0 _  g
      541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
$ ^, j  O0 R8 r& ?! s) T      601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
1 y1 U5 B4 G/ j# f$ |. e0 }6 t- M+ X      661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
# p1 ~: V' B2 }6 b, B, y6 c      721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
9 c8 ?# u* ?0 G6 c: H      781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
) q! J; H2 [4 W, K! o  f( t5 @) y      841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
1 \2 U* k, p' e9 N) n$ ^1 M      901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac7 I) L  T- j* Z9 F' I
      961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
5 ~& u; R* X( ?% x     1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
: X5 T0 {- \. c" A" {% y     1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
5 a' ~& s$ g4 O& j0 d     1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
8 j0 F9 E; X9 R( g     1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg( N8 [' S7 Z- t8 d- o% o
     1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca+ k7 U/ ]" Y4 L
     1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg" c. b$ G! D* Q
     1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
0 i* {1 ]( B! i. w5 f     1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
. S/ r( d; a9 V     1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
" l, l5 X% Y" U9 z     1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc6 R3 ?# X1 _# |! S
     1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc/ n9 M2 Y6 E/ B1 p6 N
     1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
& R, X0 @5 W7 Y3 k% T     1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
- [4 L2 F# R; d     1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
3 C3 _- p) r) ^, S  U9 o3 q" b     1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
; R1 F' j  \; N! D4 F6 {! l6 \3 Z     1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
6 T0 [- O: g3 A+ u1 O     1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc% P" ^& L, \; h6 G9 t  `: _
     2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa; M5 u+ C# O5 m7 }. [9 r6 N1 [
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg  t9 Q" t3 Y! M+ D- E  ?7 J
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
4 [7 \7 ^  c, w     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag' X! i3 O$ B3 L- I, h
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta5 `. E3 S5 I& x! ~
     2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt" R2 r! T+ g* w" W- d
     2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
2 B2 _3 R4 [+ o$ @' t     2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
0 |4 k! M$ v3 F9 S4 S! V     2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa, N0 X: y; K& l( {# o( X% y- h1 x) C
     2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg7 L% Q' `, n1 c3 o, n, a9 a4 r
     2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa- P; c& Y/ K4 J( ]5 ?. G
     2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac8 g5 ]9 u% A# k$ O) Z' ?
     2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
* M% d" j. B( \8 Z7 ~, j8 O     2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga! [( v% W, h: h5 H) |* H+ b
     2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
) Y5 s: s% ~( I3 U, x     2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga+ u5 q" v% O6 I4 [0 X9 }0 |* N
     3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga3 m3 ^* u1 e1 t7 O8 h8 B( d! B
     3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt0 N' P1 B  w' e
     3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat& _% t% i& {( O% V9 W2 m
     3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac6 j5 E( `( t- X
     3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
1 F/ z; |8 ^0 ~# ]* j: u% M! |$ ?. }7 `     3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
: @; U9 [; y5 ?% Q# O+ Y" t     3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
3 f; R) I' U4 u+ Z! l0 V! g& G     3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
3 v  m; l5 V# F; R; J! E     3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg$ W5 R, Y% d+ \' Z5 w
     3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt& G  w+ d% S9 M$ U
     3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
4 M5 t& h) L3 d     3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca# o* }- x( }9 ~  _, B0 A
     3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
, R& }/ G2 p. D9 u     3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac) Z4 g! V4 j: j. K+ t- H' e+ ~
     3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc# @  O" ]$ \6 G8 X0 p" A$ [
     3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga. C5 e5 v2 K" ^) H
     3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
* K3 p0 @8 V- D4 K% X! d     4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
  D) r  z# S. Z% j. W7 X     4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
* Z: u# }! x2 ?1 C     4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc& W+ _( _* Q/ G: o; m' R# h# |% k
     4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
: F4 |4 l8 A3 M+ k: O& U& |: Q     4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
# q* H' E3 ]( d/ |% Y# `0 _2 z9 d( o     4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
9 G/ w' q6 k/ w8 o9 p5 V     4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
* l+ K. t: ~% q; ^! d6 x+ o0 e     4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat  }1 }, m1 K) P$ a
     4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa4 G6 k5 @# k6 b# x
     4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca: }: T3 v+ }% y# a# N$ H" Y) O  p
     4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
8 {6 y/ Z7 J; q4 U     4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
# s- X7 h# G$ q     4741 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag; c( P  o5 C3 V5 r( D! T; X
     4801 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt5 V; k* n/ c" _" e* T) g+ r
     4861 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
! c  N* h, m' F$ E  M/ \     4921 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
# J/ g. L6 x2 G& w: H- f     4981 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
2 r4 e5 [/ R4 l8 x( F: @     5041 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
; w# D; L+ O; H7 e- ^     5101 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
2 d6 R% q" e3 _4 W     5161 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
  H: |; ~$ P% B# y     5221 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
' y: a6 }, z- o( Z0 N     5281 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
4 n! R+ i5 G0 R  S5 g     5341 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
7 D- ?* o1 T- O2 v+ s- v     5401 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
' n, ], W, y, T  w3 ~     5461 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg' y3 x: n4 \% A, L
     5521 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
; `% d& |0 ~# O/ v# F     5581 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc& c# J" t( I$ o7 G; y1 V; S$ a
     5641 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
8 x( ]# G$ }  s1 Q     5701 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
, Z7 ], E1 q2 k: q3 |8 A     5761 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
" s3 x4 ?; _5 V6 A" P     5821 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat3 f) @" Q6 w. I. g/ ^- k7 Q
     5881 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
( `% f+ A! r5 [* M& e3 i# Z  y     5941 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
$ \$ m5 D9 A' r3 U1 @0 J' J7 H     6001 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
- v: r4 e% }  n$ a; {) M& c     6061 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg0 K- m5 L6 C" n9 F/ X
     6121 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca( d2 b  Q& ]9 x  m
     6181 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
8 V3 H  u( q3 b: F4 S! f0 T% M! G& k     6241 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
4 }# i5 u9 b8 y; I     6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
; m6 U6 g8 h9 ~7 f4 c     6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
8 @; y! v$ B- W6 Z+ t2 Z+ c     6421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
4 K9 d- o/ x) y) X, U" A     6481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa9 P' T2 a6 d+ B7 `
     6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg9 ?6 T+ U3 z) e  Q! @) p% r; h
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg% P; f1 I, H; D/ o  V0 H
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag+ ?6 ], j# D. C$ d4 R. w9 Q
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
* E( M# ~( L' J% f$ F* [( C     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct" ]5 m2 w' g* S. C) R! x
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
) v+ m7 i' N& A, u* K. q     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg  u; B! L2 w: u% a
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
4 R% U# p/ H; K3 l5 {* S     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca* t1 N- T& ]1 s" m+ h
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
% G( n& ?6 e3 }% B" Y+ \     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
# N$ w; B) y% T; Z+ C* }% R. T7 n     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc: d: G' T. ?+ ~$ Y1 w# Z( S5 r9 j7 `
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
* D; ]4 \7 }3 ~& x  o0 ~+ H     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca6 B3 b! k4 S  N  V: O
     7381 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa" r3 h" S" i; c% q- }
     7441 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac7 }" |. G6 {5 P0 A, F
     7501 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
/ u+ V1 ?4 R( r2 M# I3 q+ T- y     7561 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
, v) U/ v0 N5 t# q     7621 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
# X- \7 @2 H% Z9 k; [5 B     7681 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg4 Q, t* o' `) S; O
     7741 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
; G* J0 d8 E+ w$ v. w4 B* N$ P     7801 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc9 K2 |2 M# O3 W7 `
     7861 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg% O6 v! _. q2 @: U3 K. d: e3 \# }
     7921 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
) L- k+ f# r: h! X/ O8 @# R     7981 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca/ k5 o/ w0 Y) i% ^( V  q
     8041 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
" G1 Y: g/ y! c$ V9 o+ Q: N$ s     8101 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat5 X+ @' {! Y1 x8 O% J8 o0 u
     8161 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
1 y7 A; t- M8 X- A& U  @' Z  |     8221 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
* D  d+ R$ E1 s* \; v7 _2 i; N     8281 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt" R6 W( j" u' g4 V: A8 k7 E
     8341 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac4 n, {) w4 g# H) F; N
     8401 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
  c  d( B7 m. X& o& B  u2 m2 {" H     8461 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
6 _8 x$ u! f6 R0 s     8521 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc' Q! X1 o" Y: u  i* c3 ~1 y* U
     8581 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
) h  R' O  I; E" G8 k! k. q     8641 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt1 J: ~+ s6 R3 x  Q
     8701 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg% z* \+ N, \$ z( e9 j8 J" P0 U
     8761 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
7 o0 c$ n0 B. E$ S7 D; e     8821 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg& C7 [* Q7 I4 R: L9 s' k# q
     8881 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
: I$ R: @3 |0 }) T     8941 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga# J. k; M" s/ j" `% n$ h$ ?. w
     9001 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
- G2 W1 T# N, F% @: i3 C) \     9061 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
& M; P+ z* y. M* z+ N     9121 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat2 d$ L: }: P3 y! K
     9181 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
, \& ?. y) m- _- {" \     9241 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg( S* U- K7 J: o' a( X4 I5 e
     9301 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca( y! P2 H3 B) L6 H
     9361 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
: K7 Q2 \  y2 W1 v( m7 P     9421 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca% A& t2 s0 I6 u3 l7 z$ K0 @
     9481 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag# k4 |: M/ ]& b9 v& U5 E$ A
     9541 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg; i5 x& C4 q  @+ C( j. M+ ]
     9601 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt5 ?3 X* L! @$ o
     9661 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
( w: Q0 n" J- m" T: P     9721 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
6 L" J1 D% V" n; L" \1 D' X     9781 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc6 [$ O) g" R. o( P, c
     9841 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
0 p  Q" M, E1 Z+ n/ N     9901 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
4 j8 u4 }$ _# }( a     9961 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt# {0 O0 o! |0 R2 \+ g0 @
    10021 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
# L+ o( \0 \. h) s. }    10081 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
# Y: a* ]+ P8 f* R    10141 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc& V9 [; o9 D3 g" [( W$ {
    10201 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct4 a7 f) I( ^  P- z0 V  Y+ U7 M
    10261 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
9 Z/ k0 k4 x# @; q    10321 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt- r/ J$ w. ~1 D+ k$ l
    10381 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga) t1 V1 z9 t# l; X$ b
    10441 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg3 J* B0 }& x5 ~) L
    10501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt" Y) n. J8 S' P; S+ d4 B
    10561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
- C  X, q, s; t. a% R    10621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga, B! U/ N7 ?$ T+ T" U
    10681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
; W2 I. K5 ]1 I% ]; f    10741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
' |; T7 u" H: v4 `7 ~" Y8 f% z    10801 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
+ |. b: c( Y" K7 P" P# u/ ]    10861 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta8 N/ P: s! t4 J- {( O0 {+ `
    10921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg# n7 P- C5 H9 C' m( s* ^
    10981 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga
. k" a6 O' _* F, M- J! ~3 d) X    11041 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
4 N% P2 {5 `) }2 ]; ?    11101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag% D6 B4 a* [' F5 S
    11161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
9 l7 J3 C3 ?, u6 ^# E! e0 ]    11221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg2 m7 }1 r  ~' O$ Q8 \2 n; F7 w3 Z& D
    11281 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
7 @$ i7 d! [0 {; `) _  A% G    11341 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
" z5 I5 W1 t% @7 t; l9 R    11401 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
0 Q5 ~2 C( ^- v7 ?' A7 B    11461 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc6 C: m5 {0 L2 v3 H0 w4 f
    11521 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
% g2 [( {$ K- q# d8 G    11581 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg. h" ?; j* `: Y, Z
    11641 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
6 m! C' O6 X3 o( j* E+ t" [' ?6 o    11701 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
  f6 g& n8 J* X' a1 E* h" ^4 v7 Q    11761 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
6 X/ P3 D; W- _6 I- C    11821 tggagagaac acgggggact cttgac

1305有啥好的，比1304好在哪了，谢谢 请告诉无知的我

1304 的信息如下! e* F8 @2 @5 D% q2 s4 v3 T
hptII $ o) z1 t- g$ }% P9 E5 X
kan1 m, t2 N3 w( _% ]( p; H& e
pUC186 {/ j, P0 Q  x6 Y! |+ G) S' U- e
gfp:gusA
4 ?' o- Y7 R" v$ i" \2 q 6128
) s+ W; Y' `4 M/ H" |! ]5 }, C参考http://www.genecool.com/bbs/thread-11887-1-1.html

好东西  thanks