pET27-b
载体编号：P0110
载体名称：
pET27-b
载体抗性：Kanamycin
载体大小：5414bp
载体宿主：E.coli
载体特点：
The pET-27b(+) vector (Cat. No. 69863-3) carries an N-terminal pelBsignal sequence for poten-(R) tial periplasmic localization, plus optional C-terminal HSV*Tag(R) and His*Tag sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs.ampicillin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
网址链接：
http://www.merckbiosciences.com/docs/docs/PROT/TB073.pdf
该资源由“ouclhf”酷友共享，特此感谢！
