pGADT7
载体编号：P0099
载体名称：pGADT7
载体抗性：氨苄
载体大小：8000bp
载体宿主：大肠杆菌 酵母
载体特点：酵母双杂交质粒
pGADT7 is the AD Vector included with Matchmaker(TM) Two-Hybrid System 3. The MCS of pGADT7 has unique restriction sites in frame with the 3'-end of the GAL4 AD for constructing a fusion protein with either a protein of interest or a fusion protein library. The bait protein is also expressed as a fusion to a hemagglutinin (HA) epitope tag. HA-tagged proteins can be identiied with antibodies raised to this common epitope, eliminating the need to generate speciic antibod-ies to new proteins. The T7 promoter is used for in vitro transcription and translation of the epitope tagged fusion protein and also provides a binding site for sequencing using the T7 Sequencing Primer. Note that the
AD is not expressed during the in vitro transcription and translation reactions.The Nco I and Pst I sites may be used to shuttle inserts from pGADT7 into pGBKT7, the Matchmaker Two-Hybrid System 3 DNA-BD Vector. The MCS in pGADT7 is compatible with those in pMyc-CMV and pHA-CMV, Clontech's epitope tagged mammalian expression vector set (Cat. No. 631604). As a result, the target gene can be shuttled into these vectors in order to conirm protein interactions in vivo.
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http://www.clontech.com/products/detail.asp?tabno=2&catalog_id=630442&page=all
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