pTrcHis C
载体编号：P0098
载体名称：pTrcHis C
载体抗性：氨苄
载体大小：4400bp
载体宿主：ECOLI
载体特点：The pTrcHisvectorsare pBR322-derived expressionvectorsdesigned for efficient Introductionrecombinant proteinexpressionand purificationin E. coli. Highlevelsofexpressionare possible usingthe trc (trp-lac)promoter(Egonet al., 1983)and the rrnB anti-termination region(Li et al., 1984). The trc promoter containsthe -35 regionofthe trp promoter together withthe -10 regionofthe lac promoter (Brosius et al., 1985; Egonet al., 1983; qMulliganet al., 1985). The pTrcHisvectorsalso containa copyofthe lacIgene whichcodesfor the lac repressor protein. Thisallowsfor efficient repressionoftranscriptionofq+ q-the cloned insert inE. coli regardlessofwhether the strainislacIor lacI .When expression is desired, the E. coli are grown to mid-logphase and IPTG (isopropyl- β-D-thiogalactoside) isadded to 1 mM to induce expression(via de-repression). Translationisenhanced by the presence ofa minicistronthat provideshighlyefficient translational restart into the openreadingframe (ORF) ofthe multiple cloningsite (MCS). DNAinsertsare positioned downstreamand inframe witha sequence that encodesanN-terminal fusionpeptide. The N-terminal peptide codesfor (5to 3fromthe promoter) anATG translationinitiationcodon, sixhistidine residuesinseriesthat functionasa metal bindingdomaininthe translated protein, the bacteriophage T7 gene
(TM)10 translationenhancer, the Xpressepitope, and anenterokinase cleavage recognitionsequence.
网站链接：
http://www.invitrogen.com/content/sfs/manuals/ptrchis_man.pdf
该资源由“ouclhf”酷友共享，特此感谢！
