谢谢flash关心哈。我是不敢忘记的，最近又点忙，所以、、、、
似乎有找借口的嫌疑阿。
今天介绍的这篇文献，我也才大概看了一下，不过感觉很不错，跟以前看过的文献相比，呵呵，杰作阿。
还是先把题目写下来：Three-step purification of lipopolysaccharide-free, polyhistidinetagged recombinant antigens of Mycobacterium tuberculosis
摘要：
Previous work has shown that the study of host immune responses against Mycobacterium tuberculosis, the causative
agent of tuberculosis, requires the availability of multiple mycobacterial antigens. Since purification of protein from M.
tuberculosis cells is extremely cumbersome, we developed a protocol for purifying milligram amounts of ten recombinant antigens of M. tuberculosis from E. coli cells. Purified proteins were immunologically active and free of contaminants that confound interpretation of cell-based immunological assays. The method utilizes a three-step purification protocol consisting of immobilized metal-chelate affinity chromatography, size exclusion chromatography and anion-exchange chromatography.The first two chromatographic steps yielded recombinant protein free of protein contaminants, while the third step (anion-exchange chromatography) efficiently removed E. coli lipopolysaccharide, a potent polyclonal activator of lymphoid cells. The recombinant proteins were immunologically indistinguishable from their native (i.e., purified from M.tuberculosis) counterparts. Thus the method provides a way to utilize recombinant proteins for immunological analyses that require highly purified antigens. Ó 1998 Elsevier Science B.V. All rights reserved.
尤其向大家推荐看一下文章的结论部分，作者对为什么选择这样的纯化策略做了详尽的分析，读了受益匪浅阿。从这篇文章中我们可以看出根据自己的试验目的和要求（即自己需要的样品纯度），设计纯化方案。
如果有兴趣大家把读后的感想说一说，讨论讨论，也可以说一说自己在做纯化时，一般根据什么来设计纯化策略，如何优化以达到最大的效果。
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