再来一篇02年的英文综述:
Practical considerations in refolding proteins from inclusion bodies
摘要部分:
包涵体蛋白的复性受几个因素的影响,包括包涵体在变性剂中的溶解能力、变性剂的去除及帮助复性的小分子添加物。本文将对以下几个关键参数进行综述:(1) conformation of the protein solubilized from inclusion bodies, (2) change in conformation and flexibility or solubility of proteins during refolding upon reduction of denaturant concentration, and (3) the effect of small molecule additives on refolding and aggregation of the proteins.
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