Rosseta gammi (DE3)
菌株编号：B0025
菌株名称：Rosseta gammi (DE3)
菌株抗性：CamR, KanR, StrR, TetR
菌株特点：
Rosetta-gami™ host strains are Origami derivatives that combine the enhanced disulfide bond formation resulting from trxB/gor mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with the plasmids carrying kanamycin or tetracycline resistance genes.
DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
网站链接：
http://www.merckbiosciences.com/product/71055
此资源由“ouclhf”酷友共享，特此感谢！
