Origami(DE3)
菌株编号：B0024
菌株名称：
Origami(DE3)
菌株抗性：Kanamycin (15 μg/ml), Streptomycin (50 μg/ml), Tetracycline (12.5 μg/ml)
菌株特点：
Origami(DE3)™ host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (
trxB) and glutathione reductase (
gor) genes, which greatly enhance disulfide bond formation in the
E. coli cytoplasm. Studies have shown that expression in Origami(DE3) yielded 10-fold more active protein than in another host even though overall expression levels were similar. The original Origami strains are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, since the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. The
trxB and
gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only be selected with kanamycin or tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in
trxB and
gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.
DE3 indicates that the host is a lysogen of
lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the
lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: D(ara-leu)7697 DlacX74 DphoA PvuII
phoR araD139 ahpC galE galK rpsLF′[
lac+ lacIq pro] (DE3)
gor522::Tn
10 trxB (KanR, StrR, TetR)
Guaranteed Efficiency: Guaranteed efficiency: > 2 x 106 cfu/
mg
网站链接：
http://www.merckbiosciences.com/product/70627
