题目【资源交换】20071112-pCI-neo-botaofengpeng-P0039 pGAPZaA帖子内容: 基因酷,您好!我是基因酷botaofengpeng,我已认真阅读基因酷产品转让协议(GC MTA)及《资源保证协议》,并同意签署。我欲以pCI-neo质粒交换基因库pGAPZaA质粒。请予以回复! 我预共享的质粒基本信息:
质粒需提供如下信息:
载体名称:pCI-neo
载体抗性:Amp
载体大小:5.4kb
载体宿主:E.coli, Mammalian cells
载体特点:The pCI-neo Vector was designed for high-level, constitutive expression of cloned DNA inserts in mammalian cells. The vector carries the cytomegalovirus (CMV) immediate-early enhancer/promoter, simian virus 40 (SV40) late polyadenylation signal and an optimized chimeric intron for regulating expression. The pCI-neo Vector also contains the neomycin phosphotransferase gene for selection of stably transfected clones, a multiple cloning region, T7 and T3 RNA polymerase promoters*, the f1 origin of replication and a highcopy prokaryotic origin of replication for E. coli.
网站链接:
http://www.promega.com/search/Default.aspx?query=pCI-neo&x=19&y=14
附件信息:附带载体的质粒图谱;