pADANS 质粒信息与图谱
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4 A. n9 k" ]' F ?4 w 来源：
http://www.informaxinc.com/view/viewer.html
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0 q0 v1 ?; F) D5 U& M6 j 抗性：ampR
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. Z0 e) ` ~, t0 j/ t8 L6 T 酵母表达载体
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promoter：yeast ADH1 promoter
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质粒图谱：
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载体序列：
9 X8 s w2 E' }! M K; ^3 H4 C- V0 o 1 agcttgcggc cgcggccttt ttggccagct cgaattcagc tttggacttc ttcgccagag
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61 gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa
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121 agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt
$ [; u# U$ l0 ^: y
181 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
+ R( K% I9 ?, h8 V6 t 241 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
, M7 b6 s3 A. n7 N ?
301 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg
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361 gcatgccgag caaatgcctg caaatcgctc cccatttcac ccaattgtag atatgctaac
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421 tccagcaatg agttgatgaa tctcggtgtg tattttatgt cctcagagga caacacctgt
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481 tgtaatcgtt cttccacacg gatcaattcg taatcatggt catagctgtt tcctgtgtga
: Q! U$ S. X t s
541 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
. [6 v8 g; R' b+ \: p$ F+ @2 c 601 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
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661 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
; B2 p8 I9 L1 W8 P0 g 721 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
8 }9 G6 {( ~3 u! l& J! D* n 781 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
) f8 e* M3 Z: X/ k' w2 N 841 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
* D) [$ b+ ` j! o* K
901 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
! K0 {; E: \5 D6 f. s. a 961 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
B! H) J$ z s! a2 L6 b 1021 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
! E) f( h: L- g% {+ M- E$ Y 1081 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
6 x9 B- q3 ]9 b* r' m9 N
1141 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
8 q* Q/ P& ]/ U8 }3 u' d 1201 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
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1261 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
( \& R0 B" L+ Y4 s8 a$ \ 1321 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
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1381 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
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1441 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
. u; c- j1 q% E5 K2 G) F4 ?* n% P/ M 1501 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6 F, k. f% i$ [1 p$ ~ 1561 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
2 ^( e* f D9 ]* I8 z9 x
1621 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
+ s! ]0 a& w; `2 r. ^
1681 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
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1741 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
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1801 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
$ n- I0 G/ o" z q! B 1861 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
- U4 J6 U, b8 f 1921 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
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1981 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
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2041 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
a; Q+ r2 N" h5 G+ T# b8 ~$ N% J 2101 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
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2161 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
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2221 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
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2281 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
1 i. D' ^) u- w; e 2341 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
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2401 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
: y' N# Y& }' f/ P 2461 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
2 [( M6 z# k/ h+ g) |6 E5 C 2521 cggaaatgtt gaatactcat actcttcctt tttcaatgat ctattacatt atgggtggta
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2581 tgttggaata aaaatcaact atcatctact aactagtatt tacgttacta gtatattatc
# l7 \0 w7 c" C8 v! f' p" s. J7 k 2641 atatacggtg ttagaagatg acgcaaatga tgagaaatag tcatctaaat tagtggaagc
3 Y8 I: u5 I B, g2 c 2701 tgaaacgcaa ggattgataa tgtaatagga tcaatgaata ttaacatata aaatgatgat
7 ~5 ]; E9 g, f 2761 aataatattt atagaattgt gtagaattgc agattccctt ttatggattc ctaaatcctc
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2821 gactacgtcg ttaaggccgt ttctgacaga gtaaaattct tgagggaact ttcaccatta
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2881 tgggaaatgg ttcaagaagg tattgactta aactccatca aatggtcagg tcattgagtg
/ L( D$ {0 N# r* o. L. {. ^ 2941 ttttttattt gttgtatttt ttttttttta gagaaaatcc tccaatatat aaattaggaa
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3001 tcatagtttc atgattttct gttacaccta actttttgtg tggtgccctc ctccttgtca
. g! m0 j" i; c- v 3061 atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag tatcaatttg
. |; h) b: T! ^ 3121 cttacctgta ttcctttaca tcctcctttt tctccttctt gataaatgta tgtagattgc
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3181 gtatatagtt tcgtctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat
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3241 tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag
6 P% I! d5 D) d; c8 c! ~; H 3301 caaggatttt cttaacttct tcggcgacag catcaccgac ttccgtggta ctgttggaac
% E+ J9 i: W+ W 3361 cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg
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3421 ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag
, f( z" |0 S" k1 M" U" K 3481 tggcgatagg gttgacctta ttctttggca aatctggagc agaaccgtgg catggttcgt
$ F$ O5 D( e1 j8 \9 Q" n 3541 acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac
. Z; X1 n* Z; s, U/ p% f 3601 ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg
, ? j7 X1 ^& w# x) ~ 3661 tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa
4 {; A, }( _$ |0 F1 r 3721 tcaattgatg ttgaaccttc aatgtaggga attcgttctt gatggtttcc tccacagttt
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3781 ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg
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3841 gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa
# Y) G: B' ]/ W6 S0 C* x5 d0 w 3901 cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa
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3961 agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg
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4021 gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg
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4081 cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta acactaccgg
$ N7 o( L- x2 E2 |! d. T 4141 taccccattt aggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt
E+ C( E+ a' ]1 b/ K. e9 P+ B, p7 o 4201 ccagcgcctc atctggaagt gggacacctg tagcatcgat agcagcacca ccaattaaat
! m( ]9 o) y% n 4261 gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa
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4321 tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag
: I+ |7 f# ^- J" `7 O 4381 gggcagacat tagaatggta tatccttgaa atatatatat atatattgct gaaatgtaaa
- U( l8 @* e( y* q- \" I 4441 aggtaagaaa agttagaaag taagacgatt gctaaccacc tattggaaaa aacaataggt
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4501 ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag catttagtca tgaacgcttc
- s! Q9 v+ u) U# x' `$ \! a 4561 tctattctat atgaaaagcc ggttccgcgg ctctcacctt tcctttttct cccaattttt
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4621 cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat
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4681 cgaatttgat tctgtgcgat agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata
: K4 Z: t+ G, l6 U2 Z 4741 atggttgcta agagattcga actcttgcat cttacgatac ctgagtattc ccacagttaa
' M; a8 X0 N2 P5 p! g2 X 4801 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
8 X7 S$ l5 @5 y) U. F% a 4861 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
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4921 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
# i: D: V- W) u8 Q7 k7 R 4981 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
+ j/ J: [8 ]4 ]6 \: l 5041 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
* k' E/ U' N6 w9 `* {+ }" o9 u
5101 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
) ~- c. T1 Q' _: c# f 5161 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
1 j: c- v: Y, o- | 5221 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
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5281 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
; I8 f' a) h: G5 C2 a l8 n 5341 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
+ t$ S: B9 D" x
5401 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
% |9 T: C& S$ P' X
5461 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
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5521 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
2 |8 g( G2 b) {( X$ d 5581 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
# u8 l' l& T$ ^0 w3 n8 z* s+ Y' J/ s
5641 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg
/ t; H( b8 O% N& f- S% q 5701 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
# X* h6 s1 d1 P* y
5761 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
# s) U+ q, M1 E A3 i) @ 5821 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
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5881 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
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5941 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
* k s& \3 i; ~& y
6001 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
3 Y3 z' F1 D7 T) h# ]/ e& |. W: n9 |% s 6061 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
3 @$ x' V5 Q3 ^, }" a
6121 catttccttt gatattggat catatgcata gtaccgagaa actagtgcga agtagtgatc
3 |0 I2 E W+ H 6181 aggtattgct gttatctgat gagtatacgt tgtcctggcc acggcagaag cacgcttatc
0 U! J5 ] a# ?5 [# ]9 R
6241 gctccaattt cccacaacat tagtcaactc cgttaggccc ttcattgaaa gaaatgaggt
S0 b" g/ @( a6 k- H# S 6301 catcaaatgt cttccaatgt gagattttgg gccatttttt atagcaaaga ttgaataagg
3 z8 }+ c; ?: N8 Q
6361 cgcatttttc ttcaaagctg gatccgaaga aatgatggta aatgaaatag gaaatcaagg
) y5 |4 V' b/ r! t 6421 agcatgaagg caaaagacaa atataagggt cgaacgaaaa ataaagtgaa aagtgttgat
4 C& N+ B. ?1 p/ P' G# g 6481 atgatgtatt tggctttgcg gcgccgaaaa aacgagttta cgcaattgca caatcatgct
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6541 gactctgtgg cggacccgcg ctcttgccgg cccggcgata acgctgggcg tgaggctgtg
$ W- J& v( E- |1 N" b" X
6601 cccggcggag ttttttgcgc ctgcattttc caaggtttac cctgcgctaa ggggcgagat
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6661 tggagaagca ataagaatgc cggttggggt tgcgatgatg acgaccacga caactggtgt
8 p) d9 b/ U+ E J+ P) ?/ @
6721 cattatttaa gttgccgaaa gaacctgagt gcatttgcaa catgagtata ctagaagaat
% j `$ ~ K7 E/ X& c9 T, @# B 6781 gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc gaccatgacc
$ Q+ B/ X% T$ l 6841 ttgaaggtga gacgcgcata accgctagag tactttgaag aggaaacagc aatagggttg
9 u8 c5 T0 O9 B' o2 G
6901 ctaccagtat aaatagacag gtacatacaa cactggaaat ggttgtctgt ttgagtacgc
7 e7 \6 d/ h3 W: X 6961 tttcaattca tttgggtgtg cactttatta tgttacaata tggaagggaa ctttacactt
) j, l! X/ u7 M, @+ j6 } 7021 ctcctatgca catatattaa ttaaagtcca atgctagtag agaagggggg taacacccct
2 j! V$ ]3 Q7 ]
7081 ccgcgctctt ttccgatttt tttctaaacc gtggaatatt tcggatatcc ttttgttgtt
' g4 s; A' |# e% u j
7141 tccgggtgta caatatggac ttcctctttt ctggcaacca aacccataca tcgggattcc
7 e5 i$ X n. a* ?+ c0 u 7201 tataatacct tcgttggtct ccctaacatg taggtggcgg aggggagata tacaatagaa
6 G W/ X* f0 l' z 7261 cagataccag acaagacata atgggctaaa caagactaca ccaattacac tgcctcattg
7 w' [+ d$ t6 J# ? w, J4 o
7321 atggtggtac ataacgaact aatactgtag ccctagactt gatagccatc atcatatcga
8 I" F7 g0 z1 Z4 h9 Z- p# [6 @% b 7381 agtttcacta ccctttttcc atttgccatc tattgaagta ataataggcg catgcaactt
/ N! X& s% q8 Q! R
7441 cttttctttt tttttctttt ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca
' i) n& Q! n. |9 D
7501 aaaaaaatga tggaagacac taaaggaaaa aattaacgac aaagacagca ccaacagatg
' {, |, s9 ]) @& k/ d
7561 tcgttgttcc agagctgatg aggggtatct tcgaacacac gaaacttttt ccttccttca
, v+ @. V! L% G* l+ @
7621 ttcacgcaca ctactctcta atgagcaacg gtatacggcc ttccttccag ttacttgaat
n2 ^+ H6 X$ b
7681 ttgaaataaa aaaagtttgc cgctttgcta tcaagtataa atagacctgc aattattaat
- s- x& e) c" p8 `
7741 cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat
5 Q* Z; D$ G$ Q 7801 atttcaagct ataccaagca tacaatcaac tatctcatat acaatgtcta tcccagaaac
E& ~/ @6 ~$ {1 c3 |( I- @
7861 tcaaaaaggt gttatcttct acga
& u C, w. N/ h& D- L% U
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本帖最后由 wcg129 于 07-10-23 16:37 编辑 ]
