载体名称：p34E-Tp
+ Z0 C8 J- {: x Z' A$ d
4 L& I9 i. E7 b+ i2 H misc_feature 1575
8 ^8 _7 i' c+ P /note=" SP6 transcription initiation site "
6 v. E8 u. h# x) A* K$ Z
/label=SP6_T/C_INIT
1 G7 }! D5 w8 Q6 w
promoter 1557..1574
- H2 b! O) h( H1 |/ g /note=" SP6 promoter "
' H6 J; f4 k* _4 a8 H /label=SP6_P
% o8 b% |( f5 Z/ c$ v
CDS complement(1638..1874)
# ?; i7 g: Q1 Y2 |# y
/label=Trimethaprim\resistance
' \5 }$ Q; F# J! d% z' z misc_feature 2306
* M' c4 @' J% [6 A& b7 G, } /note=" T7 transcription initiation site "
5 y$ Z- l+ i3 D' c0 {8 t /label=T7_T/C_INIT
* C4 D9 p) ?9 K6 D promoter complement(2301..2323)
& Z3 R+ l/ G. H/ Q* A /note=" T7 promoter "
) _9 i! E* O5 H. |. o
/label=T7_P
9 e$ c% i4 v+ R4 N6 L misc_feature 2334..2392
5 Q9 p% Z/ ?' k4 C1 U- ?0 u
/note=" lac operon sequence "
4 E9 r* U5 V: Q
/label=LAC_OPERON
9 V$ b' ]' j" a CDS complement(3334..667)
+ \: d a* [6 X: [ /label=AP(R)
8 p9 P# f5 E Z( a( F; N6 \7 s来源：
http://www.informaxinc.com/view/viewer.html3 H3 ?9 |5 ~ C; J/ g8 A
. r& e/ p$ O% U, Z _序列如下：
' z+ i: D( f; f! _8 J4 h, Z$ V
1 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
" F0 u! p# M/ o" ]6 D
61 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
8 R e) o# n5 `) E& V7 ?4 Y! P5 t2 a 121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
& L+ `- _: {+ }" l* J! h
181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
# w& v/ a6 J' |2 C5 ~) }4 D
241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
) F" x* S7 I: a1 Q1 `( X 301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
) E! b1 Q; O; H8 Q* z 361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
2 ~- b' h! d+ W$ B8 Y
421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
( s. B! v8 N/ v+ B, a
481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
. Z3 p# V& v8 L4 u J1 \3 m1 g 541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
2 i3 \: P6 f$ g( I+ `- ~! J6 P# f 601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
" G# C' r) t4 }) p# o 661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
. {5 E: w, ~+ g- U5 U2 t
721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
& k# W# Q! G+ ~' x1 }. g! E
781 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
& t/ b: S) u9 w1 o6 Q) B" { 841 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
" b3 R# Y: Y. R, r3 v; @+ n
901 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
" N9 u7 H; W5 b( _1 j! c/ J
961 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
$ D; P7 v4 H2 z9 ]5 E 1021 catcagagca gattgtactg agagtgcacc atatcgacgc tctcccttat gcgactcctg
" ^; J! J9 V0 f' @4 L: I 1081 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
. m; q5 g8 ~# j0 E2 F* L7 W
1141 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
$ W# E% ]" D [& r# }* A 1201 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
2 s3 ?* r. d( e6 } }
1261 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
- T+ s7 ?1 d5 i& V) S- ^ 1321 tagaggatct ggctagcgat gaccctgctg attggttcgc tgaccatttc cggggtgcgg
( w8 ^1 n4 R0 Q' o6 k
1381 aacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta
8 `& |. `/ W k- z8 T+ c# Z4 F- D( t
1441 cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat
6 O. _3 x* ?6 e4 n* c 1501 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
) {) Z, o$ Z! m
1561 taggtgacac tatagaatac aagcttgcat gcctgcaggt cgactctaga ggatccccgg
' ?1 d& `, b; E# u; z 1621 gtaccgagct cgaattctta ggccacacgt tcaagtgcag ccacaggata aatttgcact
" H/ Y/ Y5 M w! S# j/ |/ B
1681 gagcctgggt gggattcgga ctcgaccgca tagccttcag gagtgagttt tgtgcaatac
, b5 j. H9 r/ a, v; K
1741 caaccgacga cttgaccctg ccaagcggca ccagatttct tgcgtacgcg atcccctaag
* x; j1 W E7 j$ U. m) h 1801 ccaaaggtgg cactcagggg aagcgcaaac tgccctgcaa cgggagcgtt ggcttcatcg
6 |5 n, k3 g* j$ }
1861 ctactttgac ccatgtcgaa tccttcttgt gaatctatta tggcgacaag caaatcgaac
; S2 D7 N+ x6 ], H6 k0 F4 I' O
1921 tctgactgcc taccccacaa caactactag aaagcaccag cacaacggct gcctaacttt
: d5 ?; J6 F8 k7 Z. Y" H3 z 1981 gttttagggc gactaccctg ctgcgtaaca tcgttgctgt tccataacat caaacatcga
9 Q4 P9 }! f9 K7 Q. g2 D* b2 Q2 t1 |
2041 cccacggcgt aacgcgctta gctagcttgg atgcccgagg ctagactgta caaaaaaaca
2 K J- q( ]1 q( @& Q
2101 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
9 c6 @# ]2 N" @4 }! c% b( [2 {' t5 D# ~ 2161 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
# l2 Z& o" T& o6 m
2221 ttatgtcaac tgggttcgtg aattcgagct cggtacccgg ggatcctcta gagtcgacct
' E. D% b4 V Q r0 }* i* V 2281 gcaggcatgc aagcttccgg tctccctata gtgagtcgta ttaatttcga taagccagct
" P" O" c. L8 @) o0 h# I0 c 2341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
1 d! k+ ?- v) B8 }
2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
. F& ~/ \" m) W/ d 2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
, Z6 o2 Z0 l/ @/ O3 G4 f 2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
7 K5 h/ d: Z R, F5 m; I 2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1 f" P- [+ `& z. {4 S
2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
* M( O9 l1 l6 j& o5 c7 S. d
2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
6 z1 [% l: ^- ^ 2761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
" y- F2 \1 Z0 S. M$ g
2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
! O/ O2 ]) h- ^' F! Y' r1 l
2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
, y, n3 g0 d# M5 A# c 2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
" a" V. W8 g4 D 3001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
6 o0 {5 d( ^5 H( y. v c 3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
Y1 r6 _" h$ u. E$ ]! q 3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1 s1 Z8 }; {0 j( e; I
3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
5 E! h5 }: d. G: u9 @, Y6 B) f2 e6 l
3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
* y2 A* c X+ h& ~4 c
3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
+ Y. S8 p' ?+ A5 G+ C
3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
: X L2 ^; x. A 3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
. H# K' ?2 S& r9 j 3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaa
) R' \! r8 ~( `, B: R3 j4 L
载体图谱如下：
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本帖最后由 小宝 于 08-7-23 11:54 编辑 ]
