质粒名称：p3-TRF2
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promoter 1：T7\promoter
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promoter 2：T3\promoter
8 R+ J' t' |- mpromoter 3：lac\promoter
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rep_origin ：pMB1\ori
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2 m+ I5 L* o( U抗性：ampR
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, t8 V9 P6 N, W序列如下：
: c7 R. Z3 n. p1 S. e 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
& a3 W V1 m3 A
61 attttttaac caataggccc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
; n7 l( T; L( {3 U+ v
121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc cactattaaa gaacgtggac
% G9 a4 F) C) E& e. s' T 181 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
& S' d7 P. W. K& S" \3 x
241 cccctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
' ], O( x$ G# F+ O" X8 T7 w$ Y 301 gagcccccga tttagagctt gaacggggaa agccggcgaa cgtggcgaga aaggaaggga
" M' M Y8 ]! j" u. P' r2 I 361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agccggtcac gctgcgcgta
* Q# o7 d f% M, y$ U5 q% t" L 421 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
4 Z5 J" n8 V0 G; M9 r( Q
481 ctgccgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
; ?, _9 E" ?2 t9 p3 S 541 gaaaggggga tgtgctgcaa ggcgaattaa gttgggtaac gccagggttt tcccagtcac
5 [5 y9 I M4 a* z2 F 601 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcacttata gggcgaattg
' `/ E3 f+ @, G4 { k 661 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
& T8 k% k: A# K' Y. ?% f: Y4 n2 `' p
721 cccgggggga tccactagtt ctagatcagt tcatgccaag tcttttcatg gtccgccagc
/ A5 Q# h- T3 m5 J, N) R 781 gatccttaat catcacagct gttcggttta acaaatgggt aatttttagt aatggcagcc
0 l" S, L" N9 T" x! w 841 cagtttcctt ccccatattt ctgcactcca gccttgaccc actcgctttt cttctacagt
, }$ W: _$ ?, y `' H) b9 ]$ X6 N* k/ M
901 ccacttctgc ttttttgtta tattggttgt actgtcttca tctggtgctg cctgaacttg
$ Q F$ I" d' l2 Y" c 961 aaacagttca atcctcttcc acccaagtct ccttttcttc aaccccatta gagctgttcc
2 F$ I6 V$ ]$ r. I- s0 t
1021 acttgccttt gggtactttg ggattcttct cctccaggga ggggttggtt gagaacggtg
% P7 f& i+ ^5 G/ O
1081 ggcttggatg gtggcgctga agcggtctcc tgggaggagt tgaggcctgc gcctgggctc
3 d* `; _4 B I
1141 agtactctgg ctgtcctcct ccaagaccaa tctgcttatt gtcatgcgct tgttcttggg
& z* Q% k E: \! W. s+ j 1201 ctgcagttcc gaggccaccc tcaccgtcag ccggggctga actttcgttt tcatcttttc
1 e* B- A5 e+ u4 I5 f; J 1261 tgggtctctt gtttttgagg gctggtgatg ctggggagag cttgagtagg aagaaccaga
7 E6 _7 b5 \% F 1321 tccttctggt ccagttttgc aaaggctgcc tcagaatcct gtgcaccaga cagaggtctt
5 i- m. o$ @0 l0 m* v; C1 h 1381 gaaagctgct ttcagagtca tcattccaat ggtggttgga ggattccgta gctgccttgc
2 ^0 M+ }: p: S0 ^
1441 gggttctctg ggtggccttt tccacaggcc ctggtgctgg ctgtttatct tccttccctg
7 R" g* h& f' [ 1501 tacttgaggc agcggactca gatttcaaag ccttttttgg ccatcgtgag gaggtagggc
+ |+ a4 g9 y7 N7 [, ~2 b: S8 Y
1561 tcggcgtcat ccaggtggct ctccaggaag cgcagcatct tctgctggaa ggtctcatta
" P) w- M, r: `' Z2 X 1621 tgaaaagttc tggataacag gatgggccac gttcttttct cgaataatat tcaggagatc
+ L$ \( c+ X g 1681 atttctcagc ttctgagttt gtggggtcct tggacatatg ttttttcaaa atttttgaag
- F) `. j0 D1 e p! q
1741 ccttttcaaa ttctttgttt ttgatacaaa taatgacagc cagcttcctt gaccagtttt
J$ X) d- D/ W+ e! U2 N, r 1801 ctactggatt cgaccactgc ttctgtcagt gtaaattccg ttttaatcat ctccagcaca
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1861 tttgatagct gattccagtg gtgtgagctc agcctccata tcaaaggaac agtctaaatt
* D% G4 g, e! B5 r* Q: b 1921 ttccccttct tcaatccgcg accagacact gcataacccg cagcaatcgg gacacggtgt
# P* j9 O7 K8 ]4 k& j) m' g 1981 gctccttccc caagggcctg acaaccaaag cctgcatgat gtccccggat ctgtctgaag
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2041 tccccgtacc ggctaccccg aaaggcccgc agcgcctcgt ggaagtagaa cttgagcacc
' z, U5 J/ e8 H F ?- ? 2101 cagccgattg actgcctctt ccagccgtgc ctcccccgcg ccgcgctccg ccgggccccc
$ S# W8 {7 r- ~3 x$ G
2161 cagccccggc tcgtggcgag atctccatat gggagagctc cagcttttgt tccctttagt
) L Z& F; a9 [0 y+ Z 2221 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttccctgt gtgaaattgt
* U1 K$ d& I6 u: W/ k. T 2281 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
2 k1 _3 T1 ]* C8 c9 o4 n4 z8 C( t 2341 gcctaattga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
5 _; R/ q1 ?+ T3 `. ]0 ]
2401 gggaaacctg tcgtgccagc tgcattaaat gaatcggcca acgcgcgggg agaggcggtt
! m, u/ E% a: w: ]5 ` 2461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcc ggtcgttcgg
) r0 E* C# l6 }. D f. q 2521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
3 z! C7 G6 n2 e% N" L& c8 k 2581 gataacgcag ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
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2641 ggccgcgttg ctggcgtttt tccataggct cccgcccccc tgacgagcat cacaaaaatc
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2701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gccgtttccc
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2761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
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2821 gcctttctcc ctttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
! I) T" d8 ^3 k3 b T% ?, z3 P 2881 ttcggtgtag gtcgttcgct ccaagctggg ctgttgtgca cgaacccccc gttcagcccg
* [8 j& L' V) u. K
2941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgaactta
# }& @+ F8 d) F/ [2 {4 F4 q) d% k 3001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
. L1 ~- y0 f* m" M$ E
3061 acagagttct tgaagttggt ggcctaacta cggctacact agaaggacag tatttggtat
' ~) X9 { V8 O1 |- Z& x 3121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagaagt tggtagctct tgatccggca
$ ^4 t9 b( Q% q4 [7 V7 g 3181 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagga
; Z, j+ h9 d9 I1 s
3241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
: h3 w. ~" ]8 i3 i 3301 gaaaactcac gttaagggaa ttttggtcat gagattatca aaaaggatct tcacctagat
0 W* I1 M* q' t% |6 i1 o
3361 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt tatatatgag taaacttggt
" b: T- V* P. i: T: l 3421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
: N/ v- y7 |3 B- C3 S) L 3481 ccatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
( X" q: `# b/ Y, c: r
3541 tctggcccca gtgctgcaat gaataccgcg agacccacgc tcaccggctc cagatttatc
4 D& G J& I" V% F3 z
3601 agcaataaac cagccagccg gaagggccga gcgcagaagt ggttcctgca actttatccg
% z+ N2 x1 n. ?- D) [( S( \$ T 3661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
8 S9 L; {; G) a" t' m 3721 gttttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
( O8 c: e% c) T m 3781 atggcttcat tcagctccgg ttccccaacg atcaaggcga gttacatgat cccccatgtt
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3841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaaagt aagttggccg
0 j: N! C0 x) ~$ ]) | 3901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
0 L) s: J Y) }1 D
3961 taagatggct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
+ U+ V! F9 E5 C" s) }! t& T" u2 Y 4021 cggcgaccga gttgctcttg cccggcgttc aatacgggat aataccgcgc cacatagcag
; z9 H5 b" f) K6 r
4081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactcc tcaaggatct
# J$ e$ x) {! z( v
4141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
! n! U7 X6 Z4 G$ T+ f4 X! }3 a3 b( Z
4201 cttttacttt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
* l1 l4 z: H- m+ Z
4261 aagggaataa gggcgacacg gaaatgttga aatactcata ctcttccttt ttcaatatta
' g) `) f r5 e6 m$ w. a1 i 4321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gttatttaga
+ y: P% ?$ ~ r6 ^. N+ L9 Y 4381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
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载体图谱如下：
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本帖最后由 小宝 于 08-7-23 11:54 编辑 ]
