基因酷 基因库 Genecool » 酷友求助——保藏中心应助版 » 20080926-valeron-pAP-1-luc等8个质粒

valeron 发表于 08-9-26 18:35

20080926-valeron-pAP-1-luc等8个质粒

基因酷，您好！我是基因酷valeron，我已认真阅读《资源保证协议》，并同意签署。我欲预存：
哺乳动物双杂交系统（Promega）：pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc
信号通路报告载体（Stratagene）：pAP-1-luc，pNF-κB-luc，pSRE-luc这8个质粒。
我预共享的质粒基本信息：

载体名称：pACT
载体抗性：amp
载体大小：5566bp
载体宿主：E. coli/mammal
载体特点：The pACT Vector contains the herpes simplex virus VP16 activation domain upstream of a multiple cloning region. Two genes encoding two potentially interactive proteins of interest are cloned into the pBIND and pACT Vectors to generate fusion proteins with the DNA-binding domain of GAL4 and the activation domain of VP16, respectively.
网站链接：[url=http://www.promega.com/tbs/tm049/tm049.pdf]http://www.promega.com/tbs/tm049/tm049.pdf[/url]

载体名称：pBIND
载体抗性：amp
载体大小：6360bp
载体宿主：E. coli/mammal
载体特点：The pBIND Vector contains the yeast GAL4 DNA-binding domain upstream of a multiple cloning region. In addition, the pBIND Vector expresses the Renilla reniformis luciferase under the control of the SV40 promoter, which allows the user to normalize for differences in transfection efficiency. Two genes encoding two potentially interactive proteins of interest are cloned into the pBIND and pACT Vectors to generate fusion proteins with the DNA-binding domain of GAL4 and the activation domain of VP16,respectively.
网站链接：同上

载体名称：pACT-MyoD
载体抗性：amp
载体大小：7.3kb
载体宿主：E. coli/mammal
载体特点：The CheckMate™ Mammalian Two-Hybrid System provides positive control vectors that encode and express two proteins known to interact in vivo. MyoD is expressed in skeletal muscle and is a myogenic regulatory protein. The Id protein acts as a negative regulator of myogenic differentiation. MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector. The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA cloned in-frame with the VP16 activation domain of the pACT Vector.
网站链接：同上

载体名称：pBIND-Id
载体抗性：amp
载体大小：7.1kb
载体宿主：E. coli/mammal
载体特点：同上
网站链接：同上

载体名称：pG5luc
载体抗性：amp
载体大小：4955bp
载体宿主：E. coli/mammal
载体特点：The pG5luc Vector contains five GAL4 binding sites upstream of a minimal TATA box, which in turn is upstream of the firefly luciferase gene
(luc+).
网站链接：同上

载体名称：pAP-1-luc
载体抗性：amp
载体大小：5.7kb
载体宿主：E. coli/mammals
载体特点：PathDetect® in Vivo Signal Transduction Pathway cis-Reporting Systems组分。含AP-1增强子元件。
网站链接：[url=http://www.stratagene.com/manuals/219073.pdf]http://www.stratagene.com/manuals/219073.pdf[/url]   page 23

载体名称：pNF-κB-luc
载体抗性：amp
载体大小：5.7kb
载体宿主：E. coli/mammal
载体特点：PathDetect® in Vivo Signal Transduction Pathway cis-Reporting Systems组分。含NF-κB增强子元件。
网站链接：同上

载体名称：pSRE-luc
载体抗性：amp
载体大小：5.7kb
载体宿主：E. coli/mammal
载体特点：PathDetect® in Vivo Signal Transduction Pathway cis-Reporting Systems组分。含SRE增强子元件。
网站链接：同上

请予以回复！

保藏中心 发表于 08-9-27 21:11

您好！您的资源对于基因酷是新颖的，鉴于国庆7天假期，国庆后再寄过来吧。您还保存到有基因酷的邮寄地址么？
祝您有个愉快的国庆假期

valeron 发表于 08-9-27 21:55

好的，我会在国庆后寄出的，谢谢。

ouclhf 发表于 08-9-29 11:22

"veleron"这么热心的支持基因酷的发展，真是让人感动，建议加两点威望。

valeron 发表于 08-9-29 17:51

感谢ouclhf版主的支持！

保藏中心 发表于 08-10-4 13:20

:excellent:

valeron 发表于 08-10-7 17:26

质粒已经寄出，请注意查收。

保藏中心 发表于 08-10-9 14:13

您好！资源已经收到，我们会尽快鉴定。

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