预存质粒信息
如果缺少哪方面的资料，请随时通知我补充！请在站内短信通知我基因库地址！电脑重做系统了，以前的资料都没有了。谢谢！我会继续和基因库一起成长！
[font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]PQE40[/font][/size][/font]
[font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]amp
[/font][/size][/font][font=楷体_GB2312][size=12pt]载体大小：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]4000[/font][/size][/font]
[font=楷体_GB2312][size=12pt]载体宿主：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]E. coli[/font][/size][/font]
[align=left][font=楷体_GB2312][size=12pt]载体特点：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]pQE-40 [/font][/size][/font][font=楷体_GB2312][size=12pt]载体表达[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]DHFR [/font][/size][/font][font=楷体_GB2312][size=12pt]融合蛋白，用于短肽和低表达蛋白的稳定表达。[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]DHFR[/font][/size][/font][font=楷体_GB2312][size=12pt]能同时提高稳定性和抗原性。由于[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]DHFR[/font][/size][/font][font=楷体_GB2312][size=12pt]本身在大鼠[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]/[/font][/size][/font][font=楷体_GB2312][size=12pt]小鼠中能显示免疫力[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman], [/font][/size][/font][font=楷体_GB2312][size=12pt]因此[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]DHFR-[/font][/size][/font][font=楷体_GB2312][size=12pt]融合蛋白适用于抗原筛选。[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]载体图谱：[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]特定序列：[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]官方网站：[/size][/font][font=楷体_GB2312][size=12pt][url=http://www1.qiagen.com/Search/search.aspx?SearchTerm=pqe+40&start=0&num=50&category=0][font=Times New Roman][color=#800080]http://www1.qiagen.com/Search/search.aspx?SearchTerm=pqe+40&start=0&num=50&category=0[/color][/font][/url][/size][/font][/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][color=black][size=10.5pt][font=Times New Roman]pDsRed-Monomer-N1[/font][/size][/color][/align]
[align=left][font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]kan/neo[/font][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]载体图谱：[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]
[/size][/font][font=楷体_GB2312][size=12pt]载体大小：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]4000[/font][/size][/font][/align]
[font=楷体_GB2312][size=12pt]应[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman] [/font][/size][/font][font=楷体_GB2312][size=12pt]用：哺乳动物细胞表达[/size][/font][font=楷体_GB2312][size=12pt][/size][/font]
[size=3][font=楷体_GB2312]载体特点：[/font][/size]
[size=10.5pt]pDsRed-Monomer-N1 is a mammalian expression vector that encodes DsRed-Monomer (DsRed.M1), a monomeric mutant derived from the tetrameric [i]Discosoma sp. [/i]red fluorescent protein DsRed (1). DsRed-Monomer contains forty-five amino acid substitutions (listed on page 2). When DsRed-Monomer is expressed in mammalian cell cultures, red fluorescent cells can be detected by either fluorescence microscopy or flow cytometry 12–16 hr after transfection (DsRed-Monomer excitation and emission maxima = 557 nm and 585 nm, respectively). The DsRed-Monomer coding sequence is human codon-optimized for high expression in mammalian cells (2).[/size]
[font=Times New Roman][color=black][size=10.5pt]DsRed-Monomer is well suited for use as a fusion tag. The multiple cloning site (MCS) in pDsRed-Monomer-N1 is positioned between the immediate early promoter of CMV ([i]P[/i]CMV IE) and the DsRed-Monomer coding sequence. Genes cloned into the MCS are expressed as fusions to the N-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus sequence is located immediately upstream of the DsRed-Monomer gene to enhance translational efficiency in eukaryotic systems (3). SV40 polyadenylation signals downstream of the DsRed-Monomer gene direct proper processing of the 3' end of the DsRed-Monomer mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen, a pUC origin of replication for propagation in [i]E. coli[/i], and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neo[/size][/color][size=5.5pt]r[/size][color=black][size=10.5pt]) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of the cassette confers kanamycin resistance in [i]E. coli[/i].[/size][/color][/font]
[align=left][font=楷体_GB2312][size=12pt]官方网站：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman][url=http://www.clontech.com/products/detail.asp?tabno=2&catalog_id=632465&page=all]http://www.clontech.com/products/detail.asp?tabno=2&catalog_id=632465&page=all[/url][/font][/size][/font][/align][align=left][font=Times New Roman][size=3][/size][/font] [/align][align=left][font=Times New Roman][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][font=Times New Roman][size=10.5pt]PCAMBIA 3300[/size][font=楷体_GB2312][size=12pt][/size][/font][/font][/align]
[font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]kan
[/font][/size][/font][font=楷体_GB2312][size=12pt]载体宿主：植物表达载体系列[/size][/font][font=楷体_GB2312][size=12pt][/size][/font]
[align=left][font=楷体_GB2312][size=12pt]载体特点：关于该载体，目前已经停止销售和扩散。因为它含有一段可能扩散的基因，但是也未禁止使用（所以不知道能否预存）。详见官方网站[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]官方网址：[/size][/font][font=楷体_GB2312][size=12pt][url=http://www.cambia.org/daisy/cambia/1208.html][font=Times New Roman][color=#800080]http://www.cambia.org/daisy/cambia/1208.html[/color][/font][/url][/size][/font][/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]菌株[/size][/font][font=楷体_GB2312][size=12pt]名称：[/size][/font][size=10.5pt][font=Times New Roman]Ecoli k12 er2925[/font][/size][/align]
[align=left][font=宋体]菌株抗性[size=10.5pt] [/size]：[size=10.5pt]cam[/size][/font][/align][font=宋体][/font]
[font=宋体][/font][align=left][font=宋体][size=12pt]菌株特点：[/size][size=12pt]dam/dcm Methylation: [/size][size=12pt]dam- , dcm-[/size][/font][/align][font=宋体][/font]
[font=宋体][/font][align=left][font=宋体][size=12pt]Protease-deficient: [/size][size=12pt]No [/size][/font][/align][font=宋体][/font]
[font=宋体][/font][align=left][font=宋体][size=12pt]Laclq: [/size][size=12pt]No [/size][/font][/align][font=宋体][/font]
[font=宋体][/font][align=left][font=宋体][size=12pt]Drug Resistance: [/size][size=12pt]cam[/size][/font][/align][font=宋体][/font]
[font=宋体][/font][align=left][font=楷体_GB2312][size=12pt][font=宋体]菌株用途：甲基化酶缺陷性[/font]菌株。[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]官方链接：[/size][/font][font=楷体_GB2312][size=12pt][url=http://www.neb.com/nebecomm/products/productE4109.asp][font=Times New Roman][color=#800080]http://www.neb.com/nebecomm/products/productE4109.asp[/color][/font][/url][/size][/font][/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align][align=left][font=楷体_GB2312][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][size=10.5pt][font=Times New Roman]PBC1 [/font][/size][/align]
[align=left][font=宋体]载体大小：[/font][font=Times New Roman][size=10.5pt]21.6 kb[/size][font=楷体_GB2312][size=12pt][/size][/font][/font][/align]
[font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]amp[/font][/size][/font]
[font=楷体_GB2312][size=12pt]载体特点：[/size][/font][font=楷体_GB2312][size=12pt][/size][/font]
[align=left][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]载体图谱：[/size][/font][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt][/size][/font][/align]
[align=left][font=楷体_GB2312][size=12pt]官方网址：[/size][/font][font=楷体_GB2312][size=12pt][url=https://tools.invitrogen.com/search/index.cfm?searchterm=pbc1&fuseaction=search.simplesearch&navFilter][font=Times New Roman][color=#0000ff]https://tools.invitrogen.com/search/index.cfm?searchterm=pbc1&fuseaction=search.simplesearch&navFilter[/color][/font][/url][font=Times New Roman]=[/font][/size][/font][/align][align=left][font=Times New Roman][size=3][/size][/font] [/align][align=left][font=Times New Roman][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][font=Times New Roman][size=10.5pt]PBI221[/size][font=楷体_GB2312][size=12pt][/size][/font][/font][/align]
[font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]5667bp[/font][/size][/font]
[font=楷体_GB2312][size=12pt]载体宿主：植物表达载体系列[/size][/font][font=楷体_GB2312][size=12pt][/size][/font]
[align=left][font=楷体_GB2312][size=12pt]载体特点：详见基因库图谱库[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman] [url=http://www.genecool.com/bbs/thread-14899-1-1.html][color=#800080]http://www.genecool.com/bbs/thread-14899-1-1.html[/color][/url][/font][/size][/font][/align][align=left][font=Times New Roman][size=3][/size][/font] [/align][align=left][font=Times New Roman][size=3][/size][/font] [/align]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
[align=left][font=宋体]载[/font][font=楷体_GB2312][size=12pt]体名称：[/size][/font][size=10.5pt][font=Times New Roman]YCPLAC111[/font][/size][/align]
[font=楷体_GB2312][size=12pt]载体抗性：[/size][/font][font=楷体_GB2312][size=12pt][font=Times New Roman]amp
[/font][/size][/font][font=楷体_GB2312][size=12pt]载体特点：[/size][/font][size=11pt][font=Times New Roman]Information taken from figure 2, citation [2]. In E. coli plasmid pBR322, insert yeast genes into either EcoRI or Cla I using PolIK and dNTPs to fill in overhangs and T4 DNA ligase for blunt-end ligation. Any restriction site regenerated by this process was destroyed following digestion with the appropriate restriction enzyme, using PolIK and dNTPs to fill in overhangs and T4 DNA ligase to ligate blunt ends. Once the constructions were verified, the yeast genes were moved into the plasmid vector pUC19. Excise the gene from pBR322 by HindIII digestion, followed by mung-bean exonuclease treatment to produce blunt ends. Construct YCplac vectors (ARS1-CEN4) by blunt-end ligation of the 1.4-kb EcoRI fragment (TRP1 ARS1 genes) [3] into pBR322 EcoRI site (to make pT322) and subsequent blunt-end ligation of the functional 850-bp PvuII-HpaI CEN4 fragment [4] into pT322 ClaI (to make pTC1). Digest with AatII, and isolate the fragment containing yeast DNA from agarose gels and ligate to the large AatII-Nde I fragment of pUC19, which had the NdeI end filled in with PolIK (to make YCplac22). Make the other YCplac vectors by blunt-end ligation of the EcoRV fragment from pTC1, containing the ARS1 and CEN4 sequences, into ClaI pBR322 ClaI (to make pAC5). Insert the 1.1-kb HindIII-SmaI fragment (containing URA3) or the 1.6-kb HpaI-AccI fragment (containing LEU2) at pAC5 EcoRI as described above. Move these constructs into pUC19 as described above, to make YCplac33 and YCplac111, respectively. Construct the YEplac vector series (with a yeast 2 mu origin) by blunt-end ligation of the 1.5-kb SauIII fragment from YEp24, which contains the yeast 2 mu origin [5], into pBR322 ClaI (to make p2mu19). Remove p2mu19 XbaI site by filling in with PolIK after digestion and blunt-end religation of this site. Ligate the three yeast genes into p2mu19 EcoRI. The fragments used for the LEU2 and the URA3 constructs were the same as those used in the YCplac constructions. However, the YEplac112 vector (TRP1) contains the 850-bp EcoRI-BglII fragment of the TRP1 gene which does not contain ARS1 function. Construct the YIplac vectors by ligating each yeast gene fragment used in YEplac vector constructions into the pUC19 EcoO109 site using PolIK to fill the sticky ends. NCBI gi: 415325 Hosts: E.coli, Saccharomyces cerevisiae. Related vectors: pUC19, pBR322, yeast 2-micron plasmid, YCplac22, YCplac33, YEplac112, YEplac181, YEplac195, YIplac128, YIplac204, YIplac211.[/font][/size]
[align=left][font=宋体][size=11pt]特征序列区位：[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[table][tr][td][align=left][font=宋体][size=11pt]LEU2[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]3683 - 2589[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]pBR322_origin[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]5326 - 5945[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]Ampicillin[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]4311 - 5171[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]CEN4[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]504 - 1356[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]M13_pUC_fwd_primer[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]321 - 299[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]M13_forward20_primer[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]306 - 290[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]lacZ_a[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]287 - 442[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]M13_reverse_primer[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]206 - 224[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]M13_pUC_rev_primer[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]185 - 207[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]lac_promoter[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]142 - 171[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]AmpR_promoter[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]4241 - 4269[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]Orf2[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]4311 - 5171[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]Orf2[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][font=宋体][size=11pt]3695 - 2589[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][tr][td][align=left][font=宋体][size=11pt]TRP1(384-0)[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=left]
[font=宋体][size=12pt][/size][/font][/align]
[/td][td][align=right][align=right][font=宋体][size=11pt]2449 - 2066[/size][/font][font=宋体][size=12pt][/size][/font][/align]
[/td][/tr][/table][/align][font=宋体][size=11pt]载体图谱：[/size][/font][size=11pt][/size]
[size=11pt][/size]
[font=楷体_GB2312][size=12pt]官方网址：[/size][/font][font=楷体_GB2312][size=12pt][url=http://www.cambia.org/daisy/cambia/1208.html][font=Times New Roman][color=#800080]http://www.cambia.org/daisy/cambia/1208.html[/color][/font][/url][/size][/font]
[align=left][font=楷体_GB2312][size=12pt][font=Times New Roman][/font][/size][/font][/align]
奇怪，上面我辛辛苦苦截取的图谱怎么都没有了呢？我直接粘贴过来的
[[i] 本帖最后由 haiwuya 于 08-9-26 12:24 编辑 [/i]] :qq68: 感谢您对基因酷的支持！
这两天忙着，明天忙完了，我再看看您发上去的资料还有没有需要补充的，呵呵，我不客气的！图片也是发附件，编辑完后它会显示出来的。 要放假了，开来得国庆以后邮寄了！ 您好 ！您把图谱和序列放在附件上发。一定要有质粒图谱才行哦 收到！777777777777
页:
[1]