预存资源
[b][font=宋体][size=12pt]预存质粒[/size][/font][/b][font=宋体]基因[/font][font=宋体][size=10.5pt]酷，您好！我是基因酷haiwuya，我已认真阅读《[/size][/font][font=宋体]资源[/font][font=宋体][size=10.5pt]保证协议》，并同意签署。[/size][/font]
[font=宋体]质粒[/font][font=宋体][size=10.5pt]：Pires[/size][/font]
[font=宋体][size=10.5pt]PIRES2-EGFP[/size][/font]
[font=宋体][size=10.5pt]PQE40[/size][/font]
[font=宋体][size=10.5pt]PLNCX2[/size][/font]
[font=宋体][size=10.5pt]PDSRED-Mononer-n1[/size][/font]
[font=宋体][size=10.5pt]PCambia 3301 [/size][/font]
[font=宋体][size=10.5pt]
3300[/size][/font]
[font=宋体][size=10.5pt]Ecoli k12 er2925[/size][/font]
[font=宋体][size=10.5pt]大肠杆菌K88[/size][/font]
[font=宋体][size=10.5pt]
K99[/size][/font]
[font=宋体][size=10.5pt]
987P[/size][/font]
[font=宋体][size=10.5pt]XL1-red[/size][/font]
[font=宋体][size=10.5pt]PBI101[/size][/font]
[font=宋体][size=10.5pt]PEGFP-1[/size][/font]
[font=宋体][size=10.5pt]PSP72[/size][/font]
[font=宋体][size=10.5pt]PBC1[/size][/font]
[font=宋体][size=10.5pt]PBI221[/size][/font]
[font=宋体][size=10.5pt]YCPLAC111[/size][/font]
[font=宋体][size=10.5pt]酵母X33 [/size][/font]
[font=宋体][size=10.5pt]
KM71H[/size][/font]
[font=宋体][size=10.5pt]
GS115[/size][/font]
[font=宋体][size=10.5pt]我预共享的质粒基本信息：
[/size][/font][font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PIRES[/size][/font]
[font=宋体][size=10.5pt]载体抗性：[/size][/font][font=宋体]amp[/font]
[font=宋体][size=10.5pt]载体大小：6100
载体宿主：E. coli
载体特点：pIRES is a mammalian expression vector that allows high level expression of two genes of interest from the same bicistronic mRNA transcript. The vector contains the encephalomyocarditis virus (ECMV) internal ribosome entry site (IRES) flanked by two multiple cloning sites (MCS A and B), an arrangement that allows cap-independent translation of the gene cloned into MCS B (1–3). pIRES utilizes a partially disabled IRES sequence (1) that reduces the rate at which the gene cloned into MCS B is translated relative to that of MCS A. [/size][/font]
[font=宋体][size=10.5pt]Expression of the bicistronic transcript is driven by the constitutively active cytomegalovirus immediate early promoter ([i]P[/i]CMV IE), located upstream of MCS A. An intervening sequence (IVS) known to enhance the stability of mRNA (4) is located between [i]P[/i]CMV IE and MCS A, and is efficiently spliced out following transcription. SV40 polyadenylation signals downstream of MCS B direct proper processing of the 3' end of the mRNA. Bacteriophage T7 and T3 promoters are located upstream of MCS A and downstream of MCS B, respectively. pIRES includes a neomycin resistance gene (Neor) to aid in the selection of transfected cells. Neor is expressed from the SV40 enhancer/promoter, and a synthetic polyadenyla tion signal directs proper processing of the 3' end of the Neor mRNA. The SV40 origin al lows for replication in mammalian cells expressing the SV40 T antigen. The vector also contains an ampicillin resistance gene (Ampr), and a ColE1 origin of replication for se lection and propagation in [i]E. coli[/i], and an f1 origin for single-stranded DNA production.[/size][/font]
[font=宋体][size=10.5pt]网站链接：[url=http://www.clontech.com/products/detail.asp?catalog_id=631605][color=#800080]http://www.clontech.com/products/detail.asp?catalog_id=631605[/color][/url][/size][/font]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PLNCX2[/size][/font]
[font=宋体][size=10.5pt]载体抗性：[/size][/font][font=宋体]amp[/font]
[font=宋体][size=10.5pt]载体大小：6100
载体宿主：E. coli[/size][/font]
[font=宋体][size=10.5pt]载体特点：pLNCX2 contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression. Upon transfection into a packaging cell line, pLNCX2 can transiently express, or integrate and stably express, a transcript containing Ψ+ (the extended viral packaging signal) a selectable marker, and the gene of interest. The 5' viral LTR in this vector contains viral promoter/enhancer sequences that control expression of the neomycin resistance (Neor) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter (PCMV). pLNCX2 also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria. [/size][/font]
[font=宋体][size=10.5pt]网址链接：[url=http://www.clontech.com/products/detail.asp?product_id=10523&tabno=2][color=#0000ff]http://www.clontech.com/products/detail.asp?product_id=10523tabno=2[/color][/url][/size][/font]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PBI101[/size][/font]
[font=宋体][size=10.5pt]载体抗性：[/size][/font][font=宋体]NEO[/font]
[font=宋体][size=10.5pt]详见基因库图谱库：[url=http://bbs.genecool.com/thread-12491-1-3.html][color=#800080]http://bbs.genecool.com/thread-12491-1-3.html[/color][/url][/size][/font]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PEGFP-1[/size][/font]
[font=宋体][size=10.5pt]载体抗性：[/size][/font][font=宋体]KAN[/font]
[font=宋体][size=10.5pt]载体大小：4200
载体宿主：E. coli[/size][/font]
[font=宋体][size=10.5pt]载体特点：pEGFP-1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighterfluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emissionmaximum = 507 nm.) pEGFP-1 encodes the GFPmut1 variant (4) which contains the double-aminoacidsubstitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences (5).Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site (6)to further increase the translation efficiency in eukaryotic cells. pEGFP-1 is a promoterless EGFP vector which can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the MCS located upstream of the EGFP coding sequence. SV40polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cellsexpressing the SV40 T antigen. A neomycin-resistance cassette (Neor) allows stably transfected eukaryotic cells to be selected using G418. The Neor cassette consists of the SV40 early promoter,the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpessimplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of this cassette confers kanamycin resistance in E. coli. The pEGFP-1 backbone also provides a pUC origin[/size][/font]
[font=宋体][size=10.5pt]网址链接：[url=http://www.clontech.com/images/pt/dis_vectors/PT3026-5.pdf][color=#800080]http://www.clontech.com/images/pt/dis_vectors/PT3026-5.pdf[/color][/url][/size][/font]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PSP72[/size][/font]
[font=宋体][size=10.5pt]载体抗性：[/size][/font][font=宋体]AMP[/font]
[font=宋体][size=10.5pt]载体大小：2462[/size][/font]
[font=宋体][size=10.5pt]载体宿主：E. coli[/size][/font]
[font=宋体][size=10.5pt]载体特点：The pSP72 Vector (1) can be used as a standard cloning vector and also can be used for transcription of RNA in vitro. The pSP72 Vector contains the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning site region. [/size][/font]
[align=left][font=宋体][size=10.5pt]网址链接：[url=http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_221][color=#800080]http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_221[/color][/url][/size][/font][/align]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：YCPLAC111[/size][/font]
[align=left][font=宋体][size=10.5pt]基因库图谱库有详细说明：[url=http://bbs.genecool.com/thread-7072-1-1.html][color=#800080]http://bbs.genecool.com/thread-7072-1-1.html[/color][/url][/size][/font][/align]
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[align=left][font=宋体][size=10.5pt]菌株：XL1-RED[/size][/font][/align]
[align=left][font=宋体][size=10.5pt]特点(见说明书)：[url=http://www.stratagene.com/manuals/200129.pdf][color=#800080]http://www.stratagene.com/manuals/200129.pdf[/color][/url][/size][/font][/align]
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[font=宋体]载体[/font][font=宋体][size=10.5pt]名称：PCambia 3301 [/size][/font]
[font=宋体][size=10.5pt]质粒名称：pCAMBIA-3301
2、 载体类型：植物表达双元载体
3、 载体抗性：Kan（卡那霉素）
4、 培养条件：37度细胞培养箱
5、 其他信息：农杆菌介导,多种植物可用的双元表达载体,含有Phosphinothricin(抗固杀草除草剂)基因.[/size][/font]
[font=宋体][size=10.5pt]详细信息见基因图谱库：[url=http://www.genecool.com/bbs/thread-11887-1-1.html][color=#0000ff]http://www.genecool.com/bbs/thread-11887-1-1.html[/color][/url][/size][/font]
[font=宋体][size=10.5pt][/size][/font] haiwuya酷友，您好！Pires ，PIRES2-EGFP，PLNCX2，X33 ，KM71H，GS115基因库已经有了。一下的资源基因酷没有：
PQE40
PDSRED-Mononer-n1（您没上传资料，请补上）
PCambia 3301
3300（这是PCambia 3300么？）
Ecoli k12 er2925（您没上传资料，请补上，这个没资料，不知道是什么）
大肠杆菌
K88（您没上传资料，请补上）
K99（您没上传资料，请补上）
987P（您没上传资料，请补上）
XL1-red
PBI101
PEGFP-1
PSP72
PBC1 （您没上传资料，请补上）
PBI221（您没上传资料，请补上）
YCPLAC111 好的，我是想整理好了，发到您邮箱里！毕竟太多了，一大片，怕太乱了！好的，我马上整理！ :handshake 感谢您的配合！呵呵，发到我邮箱里，最终也得把资料放到帖子上的，资源多您也可以分开发帖
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