基因酷 基因库 Genecool » 酷友求助——保藏中心应助版 » 20080919-inankai-pQCXIH等7个质粒

inankai 发表于 08-9-19 00:16

20080919-inankai-pQCXIH等7个质粒

20080919-inankai-pQCXIH等7个质粒
基因酷，您好！我是基因酷inankai，我已认真阅读《资源保证协议》，并同意签署。我欲预存：
Renilla报告载体（Promega）：pRL-SV40, pRL-CMV
信号通路报告载体（Clontech）：pSRE-Luc
逆转录病毒载体（Clontech）：pQCXIH
哺乳动物表达载体（Invitrogen）：pEGFP-N3, pEGFP-C3
人H-Ras基因(Upstate):pUSEAmp-H-Ras (Activated form)


我预共享的质粒基本信息：
载体名称：pRL-SV40
载体抗性：amp
载体大小：3705bp
载体宿主：E. coli/mammalian
载体特点：The pRL-SV40 Vector is intended for use as an internal control
reporter and may be used in combination with any experimental reporter
vector to co-transfect mammalian cells.

载体名称：pRL-CMV
载体抗性：amp
载体大小：4079bp
载体宿主：E. coli/mammalian
载体特点：The pRL-CMV Vector is intended for use as an internal control
reporter and may be used in combination with any experimental reporter
vector to co-transfect mammalian cells.

载体名称：pQCXIH
载体抗性：amp
载体大小：7.8kb
载体宿主：E. coli/mammalian
载体特点：pQCXIH Retroviral Vector is a self-inactivating bicistronic expression vector designed to express a target gene along with the
hygromycin resistance gene. Upon transfection into a packaging cell line, this vector can transiently express, or integrate and stably
express, a viral genomic transcript containing the CMV immediate early promoter, gene of interest, IRES and the hygromycin resistance gene
(Hygr). The gene of interest and the hygromycin resistance gene are co-translated, via the internal ribosome entry site (IRES), as a
bicistronic message in mammalian cells。

载体名称：pEGFP-N3
载体抗性：kan
载体大小：4.7kb
载体宿主：E. coli/mammalian
载体特点：pEGFP-N3 encodes a red-shifted variant of wild-type GFP which has been optimized for
brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;
emission maximum = 507 nm.) pEGFP-N3 encodes the GFPmut1 variant which contains the
double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the
EGFP gene contains more than 190 silent base changes which correspond to human codon-usage
preferences. Sequences flanking EGFP have been converted to a Kozak consensus translation
initiation site to further increase the translation efficiency in eukaryotic cells.

载体名称：pEGFP-C3
载体抗性：kan
载体大小：4.7kb
载体宿主：E. coli/mammalian
载体特点：pEGFP-C3 encodes a red-shifted variant of wild-type GFP which has been optimized for
brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;
emission maximum = 507 nm.) pEGFP-N3 encodes the GFPmut1 variant which contains the
double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the
EGFP gene contains more than 190 silent base changes which correspond to human codon-usage
preferences. Sequences flanking EGFP have been converted to a Kozak consensus translation
initiation site to further increase the translation efficiency in eukaryotic cells.

载体名称：pSRE-Luc
载体抗性：amp
载体大小：5.0kb
载体宿主：E. coli/mammalian
载体特点：The Pathway Profiling Systems allow you to quickly profile the effects of a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo.Serum response element, pSRE-luc.

基因名称：H-ras （Human, activated）
载体名称：pUSEAmp
载体抗性：amp
载体大小：5.4kbp
载体宿主：E. coli/mammalian
载体特点：Human H-Ras cDNA (act)was inserted as an EcoR I fragment into pUSEamp(+). The activating mutation is a
substitution of leucine for glutamine at position 61.

inankai 发表于 08-9-19 00:17

H-Ras

保藏中心 发表于 08-9-19 14:18

您好！您预存的资源具备新颖性，可以共享。pcDNA3.1-TP53的资料不全，麻烦请您补齐了～～谢谢～！

inankai 发表于 08-9-21 09:30

野生型p53等到我拿到测序结果再说，先预存7个质粒。
预存的7个质粒已经寄出，请注意查收 顺丰速递，单号：852371920087。

保藏中心 发表于 08-9-22 08:41

好的，收到后，我们会告知您！

保藏中心 发表于 08-9-22 11:05

您好，资源已经收到！

inankai 发表于 08-10-10 19:52

保藏中心，不知道鉴定是否完成？

谢谢

保藏中心 发表于 08-10-13 09:14

您好！已经鉴定通过了，国庆放假回来我就忘了给您评分了。很抱歉！

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