基因酷 基因库 Genecool » 质粒图谱信息库 » pCAMBIA-1391Z质粒图谱及相关信息

wcg129 发表于 08-3-20 21:33

pCAMBIA-1391Z质粒图谱及相关信息

pCAMBIA-1391Z质粒图谱及相关信息
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图谱库ID：
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质粒名称：pCAMBIA-1391Z
2~e}H a+[mt+L 2g^ j,V3|;~ VS
载体类型：Bacterial
R9` s(QpR9y3~#X xq/F N&R8y*kva!N2\(Z
报告基因：GUS
1~/m"Il9x0_\k
6V(h Gi3_"xx 启动子：CAMV35S   
&t Oh1X"hw1c d9U
5s.R&rwp%E1im^4? 抗性： kan （菌）  hptII （植物）
/q4[d6B)F U,t]1d8h4b+zt
pCAMBIA-1391Z图谱1：
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[align=center][attach]5817[/attach][/align]
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{(gGD"Y/W~-g pCAMBIA-1391Z图谱2：@hD MM6i
/Z5X+ccZ
[align=center][attach]5818[/attach][/align]
F%tA9F,G4B
4N"HG8j3M3B pCAMBIA-1391Z图谱3：
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Z2Y1G3] dqlS&T [align=center][attach]5819[/attach][/align] {,vki*pE6wr Jh

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L pCAMBIA-1391Z序列及特征信息：s(Uc6F3|

,B*BC:a*uk$x LOCUS       AF234312               11226 bp    DNA     circular SYN 24-APR-2000
yL]3O!i1S J DEFINITION  Binary vector pCAMBIA-1391Z, complete sequence.1j7_N'B6w,\
ACCESSION   AF234312g5A/H5Y'xf Z6\;I
VERSION     AF234312.1  GI:7638131
U,~"pv6{+fA%G KEYWORDS    .gj.[B+m/[
SOURCE      Binary vector pCAMBIA-1391Zp
F!tXN[
  ORGANISM  Binary vector pCAMBIA-1391Z
r0C9R r;flf%CTae             other sequences; artificial sequences; vectors.W0Z4h+J1Yl!~P
REFERENCE   1  (sites)A5J A7O`[4NxW
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.5caeWOC8[
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectorsA6oMLN l8I9b
            for plant transformation
~)`5|7Hs,U{/?   JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
,z?[8mT}    PUBMED   7919218
(R;{-m4mN*W REFERENCE   2  (bases 1 to 11226)
GNc'xR   AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,HM~?U8WuI
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,?9CIvRW-{?
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
9TFt3F
y]             Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.T })o,e j
  TITLE     A comprehensive set of modular vectors for advanced manipulations#gZS7_ V]
            and efficient transformation of plants
5H5G'}Q1k9~"jC   JOURNAL   Unpublished
z%o/A%X#j ?u.H;S   REMARK    Full description of constructs
b}F _
c+f REFERENCE   3  (bases 1 to 11226)
4D(Dc+q"t!q){-S%m   AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,7VOWn%@ xU1Wy
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Xn-N&FK ^
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
{4vmh%cRwuFi%`"q             Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.b/C$J@X)@u!Z9v(U^
  TITLE     Direct Submission4sA0P7P:c0Pb2q!x2LS8tM
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /K+l$OqO9J}
            GPO Box 3200, Canberra, ACT 2601, AustraliaGNo)T\5vCd
FEATURES             Location/Qualifiersa(HVZU
     source          1..11226qYF5ot!W!GL
                     /organism="Binary vector pCAMBIA-1391Z""qzy
Fkf
                     /mol_type="other DNA"0A
qxZ/X!z
                     /db_xref="taxon:118404".n+K
q_]?Lh^
     CDS             join(2..16,207..2054) uaWD ]K
                     /codon_start=1ZB r1hj
` B
                     /product="GUSA, hexaHis tagged"
"\!EpzD+Se-S6e0E                      /protein_id="AAF65390.1"C;Me3I H]] Ug
                     /db_xref="GI:7638135"(m'SoCxiN&l
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE
z o| \8E7h                      SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
G8u
E_K2fS&dd                      GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
rN5MP8k:a6eP                      NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
p)M,],S_B%A+YB0A,|1so                      DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR
'@3g C@$t&z                      VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY(p$b.t)J!F|WEc
                     RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
^Jp^w Zd                      QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT
t _TMV*dHI;f                      CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPID3}0VZw1Y.u;n4_
                     IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ \7|'x"X8T^Q8j
                     GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV":g;xg}3H3};f
     exon            2..16
\m"D@aTks.Eo3i                      /note="synthetic first gus exon (modular)"
9tpgiYm      intron          17..206
3[$q5M PF_A}                      /note="Castorbean catalase intron; modified"^HG[im
     exon            207..2054
Q8l9A'P,[#H,c,~*[                      /note="second gusA exon, His6"
v3E1R0[ld]C [      misc_feature    2086..2338
,x*?x5R3s)lT                      /note="nos (nopaline synthase) 3'UTR (polyA signal)"
$I0]MoBvX L6X)o/m      misc_feature    2376..2401|{Y1l/@6t)c
                     /note="right border T-DNA repeat"t.YRR8{V TW"wH
     misc_feature    complement(3442..4442)
F%XsR.i                      /note="STA region from pVS1 plasmid"
Uvs-Oj l%p vU      rep_origin      complement(5035..6035)
.G{']5a6bV                      /note="pVS1-REP; replication origin from pVS1"
%c$i t]RH      misc_feature    complement(6445..6705)3w8X|7@*\2q,_*C
                     /note="bom site from pBR322"
I%M!lbg\?W9ht      rep_origin      complement(6845..7125)
"a[@1W^&T                      /note="pBR322 origin of replication"4?'u4Q%TH@
     CDS             complement(7416..8210)3vsn6bVajG7U*IT
                     /note="aadA (kanamycin resistance) gene amplified froma(R(O y,Ad^6{
                     pIG121Hm"U\ ZF!]
                     /codon_start=1
%uSZV9N                      /product="aminoglycoside phosphotransferase"
!m$x&Yt'u D7b                      /protein_id="AAF65388.1"FWM+` hpf j
                     /db_xref="GI:7638133"Q(l5dEJR
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
DEv5W+|g:k.e                      MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
^s X8Z fw)au?9x                      EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE F6na
mg H9P
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
Iz`&^0}E'dw{                      YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"o6\KwS"o
     misc_feature    8635..8660D)jF-R6Nmg
                     /note="left border repeat from C58 T-DNA"
+E5^tP"QZFh4K0F      misc_feature    8727..8935#O:bn?fe
                     /note="CaMV 3'UTR (polyA signal)"
[s[$~ z      CDS             complement(8951..9976)
s3A$~r)VlLXA                      /note="hptII (hygromycin resistance) gene"
Q S@+g P:U}
FS ~"g                      /codon_start=1
,dS%V!e"N                      /product="hygromycin phosphotransferase" l
m~(I;E|cW
                     /protein_id="AAF65389.1"
1i"e]-O#b\X4^"o
Z                      /db_xref="GI:7638134"$HI}:ImP7^#U
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
-|`;Vg(Y&{ Y9O)x(o                      GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
O2ORbn+j                      LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY6M-v3{Y ]VR$W2G;u
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
+C8t'P|1wu                      EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
Q3L-w/f;p5c#s0k                      SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS x}3f3L.d8lI%c#yv
                     TRPRAKK"
+z{a)?TG/g7[6\#nX      promoter        10928..10982PS,FoGnC&] }
                     /note="PlacZ; lacZ promoter"
y"~R ?;@ h0Rmy      CDS             11009..>11207"Zg,\ov(t`U.O
T
                     /codon_start=1+B2eP+r\;q9]
                     /product="LacZ alpha, truncated"zY d:d;x ]0S
                     /protein_id="AAF65387.1"
7jI};{Y&qp                      /db_xref="GI:7638132"
rM4Q*N"b|?                      /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPP
5o*{/_+xZ                      FASWRNSEEARTDRPSQQLRSL"
R!pi
}rW \A,HU      misc_feature    11026..110613DM*a O?*]3F3Ftg
                     /note="pUC9 MCS; polylinker"!I+BL,[+R
ORIGIN      ,pKDl2`:}0f
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct8_u:cY%o&B)Kt7C/T
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga0I%x&L"blV
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt Pf,`)~2b.[5m3f
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa;]NHc]XGE
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtgNL#M{P ?g9HZ0Jc-]
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag q-{A%\OWAX
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
P.}S$a1s @       421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg-T%@@Y#_a,u
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg'M]vINmQc
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtacn
u'X+d8c.]`gu
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
(HSY{Z:X       661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg Z}-MB2Ab*ms+m(_
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
;\Ps~X3Sz+tH       781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca@R-F&KPs
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
$tkn2RjoX$Q[| g,L       901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
hS*y1YqW{r       961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg_qdj(XuR*s\
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
4g`YG"NZ"xZ'?|      1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
K&EIcz2t7v.b\}      1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
S"L0F&H^F      1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
Iy"T?g4[Ey      1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggcaU(b*_v}R2x
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
l*X;o`&`Jc      1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
*@4kI_O3J EP9g      1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
)|O:l.c[/O      1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct:J8V*XP2u\
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttattN/z9k e EK x
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
o/g Ag4f$mO.Ls      1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata2M2n@` k
b
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat8p(J|"}A
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
)B P@2S,s      1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag.ff,xN0x
C/c
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga+q!|Rv$}Z
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
/k!h,Ji$S'W,v,J      2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
TWq4\M/s7dP2X4L-UBq      2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataatttem1F"YBO
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
6Y.lw7}jP      2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata&NdK9u4l({%r*g
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaaA ^c2E _:}/O7r
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
!aIf;^3vC'd      2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
D\"A viEjP$k6F      2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg8o`?PJ[;y.i^J
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
LA$[VQ6H.S"o      2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
np/n.B
Xr4L      2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaaZBd
Y*J'l ]3HE9g
     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
v'?"fmv.q~      2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
'b2m3kssl,F}.?      2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg_q-yca&_`0e
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
B9\y8V(s6~      2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggcfF1COE'`P-l,b
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt un)fP7`:i;z Ux-S4P
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg7f
d~k6zvOI3]
     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
6i
O;^yn;hMN,i(R      3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctcKPFj ar2RMS
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcgB/EL"~r
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga1kQ'B,FC
     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
9YP-k7\1Y?      3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac2?'ys2Gi{#YT
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggccM*I5r Gc%M:n5`BA
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
|A~$^8Msn p4]      3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
}0t%Vp5M;Ww      3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
@&IG6WA1cC      3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
A sm,x C      3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatcg*zeM.RuQ
     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcgG1z_HQ
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg7gA8HR&h
     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg$D@CL%O
     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctacAV#h` `
     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
O8mt Bn%j      4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct

V-L RP_MI      4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctgzHm(r&F9`|d-O t
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgaggK?0B^#[0O.VCU0t4Yv
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
?v6o-P[      4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
g1}P%JS-b"v      4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
W^'OQ)y      4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaatv5b8NhJ
     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag0j;OlIJ
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg wml@%M7Z[&Y#eh
     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
q$ev,N:ual \      4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtgKhHDp
     4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgccccf+L"? A/h2x/t*e
     4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
b{1C0X]7R*b7j0Ht      4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt#n1yZC&iA[7A
     4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
OG pC)K{
     5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg?f F;C2v5E#bj
     5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
0j*[}(h4O7i J_      5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
AoK8mA l^6EM      5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
n6EuRJ.t      5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac7c5?)N N?.}z
     5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggtNi+Y?V&k
     5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc Q8dy,o+S"?S6nr
     5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtgD9F5X%m-K$U5[:P
     5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
"]d,G!x(R+D jG      5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa/RHX;C:Mw)IR&uw{^
     5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg1uQjWr[8r
     5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
*t#F?qB
s
U      5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag!w+IiL7m8`
     5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
+m2zs Xg5\5z
ui      5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattgggK\
KXC,P:u)~"T){
     5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa;n2];w4s/X"e
     6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
7b6k!eef H}      6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt1eG"[9Y5y\,\ h
     6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc,rEY!lKY
     6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcgwVL N#ul
     6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
5F3dS]2xzR2lf4{      6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
(VrD st0EuC      6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
X5by9`u1D!Pr&T4Cf      6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag NG8J-|Y0D7}:f5O$f ~
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga7a
z"[*xuU8R.O(RBCCY
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgttx(Z3M)L,{J8{
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
I7t3HW'N W.@&}F
S
N9\      6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
K0nm+D-Pf      6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
AR EN.MYw      6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttcccC$b#bQo
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
a7p~0j-M%w ^.s      6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagtL9Rkv1bIl'G:ZUhU
     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac9AS u~ XWy W
     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
/A2ysIu&e*N      7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
|T ^'m`
pBT      7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
&]9d1s)`xN/I}      7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa_O3j#G)Pi'zNfqi
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa4E@#CJ0g;G/g)a$VnM
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
KG:ia4d9R3Fp5?
     7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
bw$M5UG }xY n,f      7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca?S(Xi&~K"?1d
     7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
5n~ T&uj      7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
:G'NxJ%~:tw(l,r'Dk      7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
AE l,Vs:Ph/|O[tmC
     7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt:c-^-gwPc
     7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
6\%lj8trg      7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac-IlZu4S3N
     7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa0I1SK
fw-hRVt%B
     7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
?TO%F0Y8gq      7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
5G5Dw2oh'^z P      8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt2PN5E ^:w LP2v
     8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
xi8m.@,a |*Jb:_      8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
!e6x UVn5W.dxQ      8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaactTI]*v6B-@ ]
     8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
!x|RT&}#Pm`&x/T      8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
_!] Hh8d      8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc:[R\6iPf+t'\
     8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa(lP d/K/F
     8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
4DTF%b CmMM4O%A      8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcagY(q3O_+wDrg
     8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt3Y,DIZweqR
     8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
v(M~?1T_Ed#s(q      8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
m
a?
?@hP){m"m      8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
sL0_/Tbs9c K
     8881 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatccggtcA@5T},a]
     8941 ggcatctact ctatttcttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg.E.F0Me6V&A\
     9001 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtacE Jmd)zw%j3y.N_
     9061 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
DN,hj]-r      9121 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagcaP7IR {C4ZiT
     9181 tatacgcccg gagtcgtggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
c6mI0ae,^      9241 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt!]'y9W6Pvbtc*I
     9301 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccgn9L:a)gvU#M
     9361 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct

F({j4\"@Ir Z_      9421 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
9y4R.r5N8C {/k      9481 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg^OZ?m)A[
     9541 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
*~9t(N:{*a`/UP9v      9601 cggccgcagc gatcgcatcc atagcctccg cgaccggttg tagaacagcg ggcagttcgg
xZ6_
ArL      9661 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
B_uJ z5s%o/Fh      9721 tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagtc;v4?D&g
     9781 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
W8NW'y3PP      9841 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
'Y2k"oD^"k      9901 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtgaD)Zyu0IWo5U
     9961 gttcaggctt tttcatatct cattgccccc cgggatctgc gaaagctcga gagagataga7zL,T!}d
C-m+H4M
    10021 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat;G+{?$xu1e(Rtr,to
    10081 atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga"jI;Qf2fS
    10141 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc(b7l5e4l:F
W
    10201 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatagV nHgLX)A^
    10261 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtcctttb sI`
h*]fQ
    10321 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
_-v[/v"iJ ^/O     10381 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt&c5Dv[6@
    10441 agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg
)L.Pd9t/z#c     10501 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
yX0hbFT7Fz*m     10561 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
.w8u:}7?A1@S^u     10621 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc.z9F&VM9Up hC
    10681 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg"X2p%A!{;l,\
    10741 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcca
^GB5L$A,i     10801 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
,@-`0u W9cxa     10861 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
P AuXsI#|&O|;l]     10921 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagcTu2ZqXa
    10981 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tggctgcagg
/ME8A%a3k[P;|     11041 tcgacggatc cccgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
SMPUdO H cm     11101 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
.F
x#\H&Fvmw     11161 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaagc ctagggagga
_7@oc}!@     11221 gtccac

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